Confinement induces actin flow in a meiotic cytoplasm.

Mathieu Pinot; Villier Steiner; Benoit Dehapiot; Byung-Kuk Yoo; Franck Chesnel; Laurent Blanchoin; Charles Kervrann; Zoher Gueroui

inserm-00717415, version 1

Confinement induces actin flow in a meiotic cytoplasm.

Mathieu Pinot ¹, Villier Steiner ², Benoit Dehapiot ³, Byung-Kuk Yoo ², Franck Chesnel ³, Laurent Blanchoin ⁴, Charles Kervrann ⁵, Zoher Gueroui ( Author to contact preferably ) ²

Proceedings- National Academy of Sciences Usa (2012) epub ahead of print

Abstract: In vivo, F-actin flows are observed at different cell life stages and participate in various developmental processes during asymmetric divisions in vertebrate oocytes, cell migration, or wound healing. Here, we show that confinement has a dramatic effect on F-actin spatiotemporal organization. We reconstitute in vitro the spontaneous generation of F-actin flow using Xenopus meiotic extracts artificially confined within a geometry mimicking the cell boundary. Perturbations of actin polymerization kinetics or F-actin nucleation sites strongly modify the network flow dynamics. A combination of quantitative image analysis and biochemical perturbations shows that both spatial localization of F-actin nucleators and actin turnover play a decisive role in generating flow. Interestingly, our in vitro assay recapitulates several symmetry-breaking processes observed in oocytes and early embryonic cells.

1: Compartimentation et dynamique cellulaires (CDC)
CNRS : UMR144 – Institut Curie – Université Pierre et Marie Curie [UPMC] - Paris VI
2: Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (PASTEUR)
CNRS : UMR8640 – Université Pierre et Marie Curie [UPMC] - Paris VI – Ecole normale supérieure de Paris - ENS Paris
3: Institut de Génétique et Développement de Rennes (IGDR)
Université de Rennes 1 – CNRS : UMR6290 – Biosit
4: Laboratoire de physiologie cellulaire végétale (LPCV)
CNRS : UMR5168 – Institut national de la recherche agronomique (INRA) : UR1200 – CEA : DSV/IRTSV – Université Joseph Fourier - Grenoble I
5: SERPICO (INRIA)
INRIA

inserm-00717415, version 1
http://www.hal.inserm.fr/inserm-00717415
oai:www.hal.inserm.fr:inserm-00717415
From: Hervé De Villemeur <>
Submitted on: Thursday, 12 July 2012 16:50:35
Updated on: Monday, 23 July 2012 10:07:13

See detailed view

Associated documents

PubMed: 22753521

PubMed Central: 3406835

DOI: 10.1073/pnas.1121583109

Export

Bibtex EndNote TEI RefWorks

%% inserm-00717415, version 1
%% http://www.hal.inserm.fr/inserm-00717415
@article{pinot:inserm-00717415,
    hal_id = {inserm-00717415},
    url = {http://www.hal.inserm.fr/inserm-00717415},
    title = {{Confinement induces actin flow in a meiotic cytoplasm.}},
    author = {Pinot, Mathieu and Steiner, Villier and Dehapiot, Benoit and Yoo, Byung-Kuk and Chesnel, Franck and Blanchoin, Laurent and Kervrann, Charles and Gueroui, Zoher},
    abstract = {{In vivo, F-actin flows are observed at different cell life stages and participate in various developmental processes during asymmetric divisions in vertebrate oocytes, cell migration, or wound healing. Here, we show that confinement has a dramatic effect on F-actin spatiotemporal organization. We reconstitute in vitro the spontaneous generation of F-actin flow using Xenopus meiotic extracts artificially confined within a geometry mimicking the cell boundary. Perturbations of actin polymerization kinetics or F-actin nucleation sites strongly modify the network flow dynamics. A combination of quantitative image analysis and biochemical perturbations shows that both spatial localization of F-actin nucleators and actin turnover play a decisive role in generating flow. Interestingly, our in vitro assay recapitulates several symmetry-breaking processes observed in oocytes and early embryonic cells.}},
    keywords = {cytoskeleton; self-organization; symmetry breaking},
    language = {English},
    affiliation = {Compartimentation et dynamique cellulaires - CDC , Processus d'Activation S{\'e}lective par Transfert d'Energie Uni-{\'e}lectronique ou Radiatif - PASTEUR , Institut de G{\'e}n{\'e}tique et D{\'e}veloppement de Rennes - IGDR , Laboratoire de physiologie cellulaire v{\'e}g{\'e}tale - LPCV , SERPICO - INRIA},
    pages = {epub ahead of print},
    journal = {Proceedings- National Academy of Sciences Usa},
    audience = {international },
    issn = {0027-8424 },
    doi = {10.1073/pnas.1121583109 },
    year = {2012},
    month = Jul,
}

<?xml version="1.0" encoding="UTF-8"?>
<listBibl xmlns="http://www.tei-c.org/ns/1.0" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML">
<listBibl type="article"><head>Articles in peer-reviewed journal</head>
<biblStruct type="article" xml:id="inserm-00717415"><analytic>
<author><persName><forename>Mathieu</forename><surname>Pinot</surname></persName>
<affiliation>
<orgName type="laboratory">CDC</orgName>
<orgName type="team">Compartimentation et dynamique cellulaires</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR144</orgName>
<orgName type="institution">Institut Curie</orgName>
<orgName type="institution">Université Pierre et Marie Curie [UPMC] - Paris VI</orgName>
</affiliation>
</author>
<author><persName><forename>Villier</forename><surname>Steiner</surname></persName>
<affiliation>
<orgName type="laboratory">PASTEUR</orgName>
<orgName type="team">Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR8640</orgName>
<orgName type="institution">Université Pierre et Marie Curie [UPMC] - Paris VI</orgName>
<orgName type="institution">Ecole normale supérieure de Paris - ENS Paris</orgName>
</affiliation>
</author>
<author><persName><forename>Benoit</forename><surname>Dehapiot</surname></persName>
<affiliation>
<orgName type="laboratory">IGDR</orgName>
<orgName type="team">Institut de Génétique et Développement de Rennes</orgName>
<country>FR</country>
<orgName type="institution">Université de Rennes 1</orgName>
<orgName type="institution">CNRS : UMR6290</orgName>
<orgName type="institution">Biosit</orgName>
</affiliation>
</author>
<author><persName><forename>Byung-Kuk</forename><surname>Yoo</surname></persName>
<affiliation>
<orgName type="laboratory">PASTEUR</orgName>
<orgName type="team">Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR8640</orgName>
<orgName type="institution">Université Pierre et Marie Curie [UPMC] - Paris VI</orgName>
<orgName type="institution">Ecole normale supérieure de Paris - ENS Paris</orgName>
</affiliation>
</author>
<author><persName><forename>Franck</forename><surname>Chesnel</surname></persName>
<affiliation>
<orgName type="laboratory">IGDR</orgName>
<orgName type="team">Institut de Génétique et Développement de Rennes</orgName>
<country>FR</country>
<orgName type="institution">Université de Rennes 1</orgName>
<orgName type="institution">CNRS : UMR6290</orgName>
<orgName type="institution">Biosit</orgName>
</affiliation>
</author>
<author><persName><forename>Laurent</forename><surname>Blanchoin</surname></persName>
<affiliation>
<orgName type="laboratory">LPCV</orgName>
<orgName type="team">Laboratoire de physiologie cellulaire végétale</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR5168</orgName>
<orgName type="institution">Institut national de la recherche agronomique (INRA) : UR1200</orgName>
<orgName type="institution">CEA : DSV/IRTSV</orgName>
<orgName type="institution">Université Joseph Fourier - Grenoble I</orgName>
</affiliation>
</author>
<author><persName><forename>Charles</forename><surname>Kervrann</surname></persName>
<affiliation>
<orgName type="laboratory">INRIA</orgName>
<orgName type="team">SERPICO</orgName>
<country>FR</country>
<orgName type="institution">INRIA</orgName>
</affiliation>
</author>
<author><persName><forename>Zoher</forename><surname>Gueroui</surname></persName>
<affiliation>
<orgName type="laboratory">PASTEUR</orgName>
<orgName type="team">Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR8640</orgName>
<orgName type="institution">Université Pierre et Marie Curie [UPMC] - Paris VI</orgName>
<orgName type="institution">Ecole normale supérieure de Paris - ENS Paris</orgName>
</affiliation>
</author>
<title type="main" level="a">Confinement induces actin flow in a meiotic cytoplasm.</title></analytic><monogr><title level="j" type="main">Proceedings- National Academy of Sciences Usa</title><imprint><biblScope type="publicationDate">2012-07-02</biblScope><biblScope type="pp">epub ahead of print</biblScope></imprint></monogr><idno type="doi">10.1073/pnas.1121583109</idno><idno type="HALid">http://www.hal.inserm.fr/inserm-00717415</idno></biblStruct>
</listBibl>
</listBibl>