A transgenic mouse model engineered to investigate human brain-derived neurotrophic factor in vivo

Fabrice Guillemot; Italina Cerutti; Charles Auffray; Marie-Dominique Devignes

inria-00103858, version 1

A transgenic mouse model engineered to investigate human brain-derived neurotrophic factor in vivo

Fabrice Guillemot^a^, 1, Italina Cerutti^a^, 2, Charles Auffray^a^, 1, Marie-Dominique Devignes ()^a^, 3

Transgenic research. (2006)

a – CNRS
1: Genexpress, Génomique Fonctionnelle et Biologie Systémique pour la Santé
CNRS : UMR7091 – Université Pierre et Marie Curie (UPMC) - Paris VI 7 rue Guy Moquet, BP8, 94801 Villejuif cedex France
2: Service d'Expérimentation Animale et de Transgenèse-SEAT
CNRS : UPS44 7 rue Guy Moquet, BP8, 94801 Villejuif cedex France
3: ORPAILLEUR (INRIA Lorraine - LORIA)
INRIA – CNRS : UMR7503 – Université Henri Poincaré - Nancy I – Université Nancy II – Institut National Polytechnique de Lorraine (INPL) France

Bibliographic reference

Attached file list to this document:

PDF

BDNF-TansgenicResearch.pdf

(441.7 KB)

inria-00103858, version 1
http://hal.inria.fr/inria-00103858
oai:hal.inria.fr:inria-00103858
From: Marie-Dominique Devignes <>
Submitted on: Wednesday, 22 November 2006 16:07:31
Updated on: Thursday, 23 November 2006 14:13:14

See short view

Associated documents

PDF :

Export

Bibtex EndNote TEI RefWorks

%% inria-00103858, version 1
%% http://hal.inria.fr/inria-00103858
@article{guillemot:inria-00103858,
    hal_id = {inria-00103858},
    url = {http://hal.inria.fr/inria-00103858},
    title = {{A transgenic mouse model engineered to investigate human brain-derived neurotrophic factor in vivo}},
    author = {Guillemot, Fabrice and Cerutti, Italina and Auffray, Charles and Devignes, Marie-Dominique},
    abstract = {{Brain-derived neurotrophic factor (BDNF) is an attractive component for the treatment of various neurodegenerative diseases such as Alzheimer's or Parkinson's disease. Innovative non invasive therapeutic approaches involve appropriate pharmacological induction of endogenous BDNF synthesis in brain. A transgenic mouse model has been established to study human BDNF gene expression and permit the screening of compounds capable of stimulating its activity. A 145-kb yeast artificial chromosome carrying the human BDNF gene has been engineered to produce the transgene which contains the extended BDNF promoter and 3' flanking regions and has integrated the enhanced green fluorescent protein (E-GFP) coding sequence in place of the BDNF coding exon. Five transgenic lines have been obtained through microinjection of the YAC into fertilized mouse oocytes. From the three lines expressing the transgene, one displays the specific pattern of BDNF expression. Faithful tissue-restricted transcription of BDNF 5' exons and localization of the fluorescent reporter gene product in the expected brain subregions are reported. This line constitutes an exploitable system for investigating human BDNF gene regulation in vivo.}},
    keywords = {BDNF; neurotrophic factor; YAC; transgene; expression; reporter},
    language = {English},
    affiliation = {Genexpress, G{\'e}nomique Fonctionnelle et Biologie Syst{\'e}mique pour la Sant{\'e} , Service d'Exp{\'e}rimentation Animale et de Transgen{\`e}se-SEAT , ORPAILLEUR - INRIA Lorraine - LORIA},
    publisher = {SpringerLink},
    journal = {Transgenic research.},
    note = {Association Fran{\c c}aise contre les Myopathies (AFM) , Ligue Nationale contre le Cancer , Association France-Alzheimer },
    audience = {not specified },
    year = {2006},
    pdf = {http://hal.inria.fr/inria-00103858/PDF/BDNF-TansgenicResearch.pdf},
}

%F inria-00103858, version 1
%0 Journal Article
%U http://hal.inria.fr/inria-00103858
%2 INFO:INFO_BI;SDV:BIBS;INFO:INFO_BT;SDV:BIO
%T A transgenic mouse model engineered to investigate human brain-derived neurotrophic factor in vivo
%A Guillemot, Fabrice
%A Cerutti, Italina
%A Auffray, Charles
%A Devignes, Marie-Dominique
%+ Genexpress, Génomique Fonctionnelle et Biologie Systémique pour la Santé , Service d'Expérimentation Animale et de Transgenèse-SEAT , ORPAILLEUR - INRIA Lorraine - LORIA
%X Brain-derived neurotrophic factor (BDNF) is an attractive component for the treatment of various neurodegenerative diseases such as Alzheimer's or Parkinson's disease. Innovative non invasive therapeutic approaches involve appropriate pharmacological induction of endogenous BDNF synthesis in brain. A transgenic mouse model has been established to study human BDNF gene expression and permit the screening of compounds capable of stimulating its activity. A 145-kb yeast artificial chromosome carrying the human BDNF gene has been engineered to produce the transgene which contains the extended BDNF promoter and 3' flanking regions and has integrated the enhanced green fluorescent protein (E-GFP) coding sequence in place of the BDNF coding exon. Five transgenic lines have been obtained through microinjection of the YAC into fertilized mouse oocytes. From the three lines expressing the transgene, one displays the specific pattern of BDNF expression. Faithful tissue-restricted transcription of BDNF 5' exons and localization of the fluorescent reporter gene product in the expected brain subregions are reported. This line constitutes an exploitable system for investigating human BDNF gene regulation in vivo.
%G English
%J Transgenic research.
%8 2006-00-00
%2 not specified
%I SpringerLink
%K BDNF; neurotrophic factor; YAC; transgene; expression; reporter
%2 2006
%Z Association Française contre les Myopathies (AFM) , Ligue Nationale contre le Cancer , Association France-Alzheimer

<?xml version="1.0" encoding="UTF-8"?>
<listBibl xmlns="http://www.tei-c.org/ns/1.0" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML">
<listBibl type="article"><head>Articles in peer-reviewed journal</head>
<biblStruct type="article" xml:id="inria-00103858"><analytic>
<author><persName><forename>Fabrice</forename><surname>Guillemot</surname></persName>
<affiliation>
<orgName type="team">Genexpress, Génomique Fonctionnelle et Biologie Systémique pour la Santé</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR7091</orgName>
<orgName type="institution">Université Pierre et Marie Curie (UPMC) - Paris VI</orgName>
</affiliation>
</author>
<author><persName><forename>Italina</forename><surname>Cerutti</surname></persName>
<affiliation>
<orgName type="team">Service d'Expérimentation Animale et de Transgenèse-SEAT</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UPS44</orgName>
</affiliation>
</author>
<author><persName><forename>Charles</forename><surname>Auffray</surname></persName>
<affiliation>
<orgName type="team">Genexpress, Génomique Fonctionnelle et Biologie Systémique pour la Santé</orgName>
<country>FR</country>
<orgName type="institution">CNRS : UMR7091</orgName>
<orgName type="institution">Université Pierre et Marie Curie (UPMC) - Paris VI</orgName>
</affiliation>
</author>
<author><persName><forename>Marie-Dominique</forename><surname>Devignes</surname></persName>
<affiliation>
<orgName type="laboratory">INRIA Lorraine - LORIA</orgName>
<orgName type="team">ORPAILLEUR</orgName>
<country>FR</country>
<orgName type="institution">INRIA</orgName>
<orgName type="institution">CNRS : UMR7503</orgName>
<orgName type="institution">Université Henri Poincaré - Nancy I</orgName>
<orgName type="institution">Université Nancy II</orgName>
<orgName type="institution">Institut National Polytechnique de Lorraine (INPL)</orgName>
</affiliation>
</author>
<title type="main" level="a">A transgenic mouse model engineered to investigate human brain-derived neurotrophic factor in vivo</title></analytic><monogr><title level="j" type="main">Transgenic research.</title><imprint><publisher>SpringerLink</publisher><biblScope type="publicationDate">2006-00-00</biblScope></imprint></monogr><idno type="HALid">http://hal.inria.fr/inria-00103858</idno><idno type="HALFile">http://hal.inria.fr/inria-00103858/PDF/BDNF-TansgenicResearch.pdf</idno></biblStruct>
</listBibl>
</listBibl>