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Etude génomique des mécanismes nucléaires de contrôle qualité et dégradation de l'ARN

Abstract : Eukaryotic transcription of messenger RNAs (mRNAs) is a complex multistep process. In parallel with this fundamental mechanism, many proteins will bind to the nascent mRNA in order to process and package it to form an export competent ribonucleoprotein particle (mRNP). These mRNP biogenesis steps are under the surveillance of a quality control system (QC) that will detect all the faulty events that can lead to the formation of an aberrant particle. Aberrant transcripts will be retained in the nucleus and degraded. To study the QC mechanisms, we previously implemented a powerful assay based on the global perturbation of mRNP biogenesis by the bacterial Rho factor. When expressed in the yeast nucleus, Rho will interfere with co-transcriptional mRNP assembly and generates aberrant transcripts which will be substrates for the QC and degradation system. This study extend the previous observations made by the team about implication of some proteins in the QC pathway by genome-wide methods (RNA-seq, ChIP-seq). Moreover, study of the THO complex, which is a packaging and export factor, shows that the Tho2 subunit is involved in the tagging of aberrant transcripts and in recruitment of the exonuclease Rrp6 on its targets. Finally, we are giving insights about the presence, in yeast, of a second degradation pathway for aberrant mRNPs different from the canonical pathway involving Rrp6.
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https://tel.archives-ouvertes.fr/tel-03101419
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Submitted on : Thursday, January 7, 2021 - 10:12:07 AM
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  • HAL Id : tel-03101419, version 1

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Kevin Moreau. Etude génomique des mécanismes nucléaires de contrôle qualité et dégradation de l'ARN. Sciences agricoles. Université d'Orléans, 2019. Français. ⟨NNT : 2019ORLE3012⟩. ⟨tel-03101419⟩

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