Fermentation Condition Optimization for Endophytic Fungus BS002 Isolated from Sophora Flavescens by Response Surface Methodology

Abstract : The endophytic fungus BS002 with good effect of disease-resistant has been isolated from the medicinal Sophora flavescens. The conditions of the fermentation medium were explored in this paper. On the basis of the single factor experiments, CH3COONa, potato and glucose were defined to be the main factors by Plackett-Burman Design; Response surface methodology (RSM) with Box-Behnke Design was used to optimize fermentation conditions of endophytic fungi BS002. The optimal conditions are defined as follows: 100 mL in the container 250mL, potato 246.47 g/L, glucose 27.81 g/L, CH3COONa 1.95 g/L, concentration of fungus 107 cfu/ml, temperature 25 oC, yeast extract 1.0 g/L, speed 150 rpm, fermentation for 4 d, dry weight of mycelium can reach 10.398 g/L.
Document type :
Conference papers
Complete list of metadatas

Cited literature [20 references]  Display  Hide  Download

https://hal.inria.fr/hal-01220845
Contributor : Hal Ifip <>
Submitted on : Tuesday, October 27, 2015 - 8:28:41 AM
Last modification on : Wednesday, January 17, 2018 - 10:45:35 AM
Long-term archiving on : Thursday, January 28, 2016 - 10:21:20 AM

File

978-3-642-54341-8_36_Chapter.p...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Na Yu, Lu He. Fermentation Condition Optimization for Endophytic Fungus BS002 Isolated from Sophora Flavescens by Response Surface Methodology. 7th International Conference on Computer and Computing Technologies in Agriculture (CCTA), Sep 2013, Beijing, China. pp.342-350, ⟨10.1007/978-3-642-54341-8_36⟩. ⟨hal-01220845⟩

Share

Metrics

Record views

163

Files downloads

108