 |
 |
 |
The post-transcriptional regulatory system CSR controls the balance of metabolic pools in upper glycolysis of Escherichia coli
Abstract : Metabolic control in Escherichia coli is a complex process involving multilevel regulatory systems but the involvement of post-transcriptional regulation is uncertain. The post-transcriptional factor CsrA is stated as being the only regulator essential for the use of glycolytic substrates. A dozen enzymes in the central carbon metabolism (CCM) have been reported as potentially controlled by CsrA, but its impact on the CCM functioning has not been demonstrated. Here, a multiscale analysis was performed in a wildtype strain and its isogenic mutant attenuated for CsrA (including growth parameters, gene expression levels, metabolite pools, abundance of enzymes and fluxes). Data integration and regulation analysis showed a coordinated control of the expression of glycolytic enzymes. This also revealed the imbalance of metabolite pools in the csrA mutant upper glycolysis, before the phosphofructokinase PfkA step. This imbalance is associated with a glucose–phosphate stress. Restoring PfkA activity in the csrA mutant strain suppressed this stress and increased the mutant growth rate on glucose. Thus, the carbon storage regulator system is essential for the effective
functioning of the upper glycolysis mainly through its control of PfkA. This work demonstrates the pivotal role of post-transcriptional regulation to shape the carbon metabolism.
Cited literature [49 references]
https://hal.archives-ouvertes.fr/hal-02147255
Contributor : Brice Enjalbert <>
Submitted on : Tuesday, June 4, 2019 - 3:24:57 PM
Last modification on : Tuesday, March 17, 2020 - 3:32:54 AM
Contributor : Brice Enjalbert <>
Submitted on : Tuesday, June 4, 2019 - 3:24:57 PM
Last modification on : Tuesday, March 17, 2020 - 3:32:54 AM
File
Morin Enjalbert 2016.pdf
Files produced by the author(s)
