Evolution and Design Governing Signal Precision and Amplification in a Bacterial Chemosensory Pathway

Abstract : Understanding the principles underlying the plasticity of signal transduction networks is fundamental to decipher the functioning of living cells. In Myxococcus xanthus, a particular chemosensory system (Frz) coordinates the activity of two separate motility systems (the A-and S-motility systems), promoting multicellular development. This unusual structure asks how signal is transduced in a branched signal transduction pathway. Using combined evolution guided and single cell approaches, we successfully uncoupled the regulations and showed that the A-motility regulation system branched-off an existing signaling system that initially only controlled S-motility. Pathway branching emerged in part following a gene duplication event and changes in the circuit structure increasing the signaling efficiency. In the evolved pathway, the Frz histidine kinase generates a steep biphasic response to increasing external stimulations, which is essential for signal partitioning to the motility systems. We further show that this behavior results from the action of two accessory response regulator proteins that act independently to filter and amplify signals from the upstream kinase. Thus, signal amplification loops may underlie the emergence of new connectivity in signal transduction pathways.
Document type :
Journal articles
Complete list of metadatas

Cited literature [72 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02282631
Contributor : Virginie Molle <>
Submitted on : Wednesday, September 11, 2019 - 5:11:13 PM
Last modification on : Monday, September 16, 2019 - 1:48:01 PM

File

journal.pgen.1005460.PDF
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Mathilde Guzzo, Rym Agrebi, Leon Espinosa, Gregory Baronian, Virginie Molle, et al.. Evolution and Design Governing Signal Precision and Amplification in a Bacterial Chemosensory Pathway. PLoS Genetics, Public Library of Science, 2015, 11 (8), pp.e1005460. ⟨10.1371/journal.pgen.1005460⟩. ⟨hal-02282631⟩

Share

Metrics

Record views

38

Files downloads

5