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Theses

Impact de la réticulation à la génipine sur les propriétés biophysiques d'un hydrogel d'acide hyaluronique pour la culture en trois dimensions.

Abstract : Three-dimensional (3D) biomimetic cell culture platforms offer more realisticmicroenvironments that cells naturally experience in vivo. We developed a tunable hyaluronan(HA)-based hydrogels that could easily be modified to mimic healthy or malignant extracellularmatrices (ECMs). For that, we pre-functionalized our hydrogels with an adhesive polypeptide(poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present intumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrationsof genipin (GnP). Then, we thoroughly characterized our substrates before testing them withglioblastoma, breast cancer cells and thereafter with endothelial cells and hematopoietic stemcells. Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for everypre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also withtype IV collagen but to a lesser extent. While PLL-treated hydrogels were not favorable to theculture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells ina stiffness-dependent manner. Contrary to type III collagen, type IV collagen pre-treatedhydrogels supported the proliferation of glioblastoma cells. The as-desired HA-based 3Dtumor-like models we developed may provide a useful platform for the study of various cancercells by simply tuning their biochemical composition and their mechanical properties.
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https://tel.archives-ouvertes.fr/tel-03252427
Contributor : Abes Star :  Contact
Submitted on : Monday, June 7, 2021 - 4:38:52 PM
Last modification on : Wednesday, November 3, 2021 - 5:11:18 AM
Long-term archiving on: : Wednesday, September 8, 2021 - 7:22:45 PM

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  • HAL Id : tel-03252427, version 1

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Sarah Bonnesoeur. Impact de la réticulation à la génipine sur les propriétés biophysiques d'un hydrogel d'acide hyaluronique pour la culture en trois dimensions.. Biologie cellulaire. Normandie Université, 2020. Français. ⟨NNT : 2020NORMR104⟩. ⟨tel-03252427⟩

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