Genome-scale Metabolic Reconstruction of the Eukaryote Cell Factory Yarrowia Lipolytica

Nicolás Loira 1, 2 David James Sherman 2, 1
1 MAGNOME - Models and Algorithms for the Genome
Inria Bordeaux - Sud-Ouest, UB - Université de Bordeaux, CNRS - Centre National de la Recherche Scientifique : UMR5800
Abstract : Yarrowia lipolytica is an oleaginous yeast studied experimentally for its role as a food contaminant and its use in bioremediation and cell factory applications. As one of the hemiascomycetous yeasts completely sequenced in the Génolevures program it enjoys a high quality manual annotation by a network of experts. We have developed a method of semi-automatic reconstruction of metabolic models, based on the prediction of conservation of enzymatic activities between two species. Following our reconstruction protocol, we extrapolated a Y.lipolytica genome-scale metabolic model (called iNL705) from an existing S.cerevisiae model (iND750). This draft model was curated by a group of experts in Y. lipolytica metabolism, and iteratively improved and validated through comparison with experimental data by flux balance analysis.
Type de document :
Communication dans un congrès
11th International Conference on Systems Biology, Oct 2010, Edinburgh, United Kingdom. 2010
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https://hal.inria.fr/hal-00652922
Contributeur : David James Sherman <>
Soumis le : vendredi 16 décembre 2011 - 15:37:11
Dernière modification le : jeudi 11 janvier 2018 - 06:22:12

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  • HAL Id : hal-00652922, version 1

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Nicolás Loira, David James Sherman. Genome-scale Metabolic Reconstruction of the Eukaryote Cell Factory Yarrowia Lipolytica. 11th International Conference on Systems Biology, Oct 2010, Edinburgh, United Kingdom. 2010. 〈hal-00652922〉

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