Wolbachia detection: an assessment of standard PCR protocols.

P. M. Simões 1 G. Mialdea 1 D. Reiss 1 Marie-France Sagot 1 S. Charlat 1
1 BAMBOO - An algorithmic view on genomes, cells, and environments
Inria Grenoble - Rhône-Alpes, LBBE - Laboratoire de Biométrie et Biologie Evolutive - UMR 5558
Abstract : Wolbachia is a large monophyletic genus of intracellular bacteria, traditionally detected using PCR assays. Its considerable phylogenetic diversity and impact on arthropods and nematodes make it urgent to assess the efficiency of these screening protocols. The sensitivity and range of commonly used PCR primers and of a new set of 16S primers were evaluated on a wide range of hosts and Wolbachia strains. We show that certain primer sets are significantly more efficient than others but that no single protocol can ensure the specific detection of all known Wolbachia infections.
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Submitted on : Monday, October 29, 2012 - 6:23:18 PM
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P. M. Simões, G. Mialdea, D. Reiss, Marie-France Sagot, S. Charlat. Wolbachia detection: an assessment of standard PCR protocols.. Molecular Ecology Resources, Wiley/Blackwell, 2011, 11 (3), pp.567-72. ⟨10.1111/j.1755-0998.2010.02955.x⟩. ⟨hal-00746848⟩



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