Abstract : According to recent literature, the most appropriate preprocessing to improve P300 detection is still unknown or at least there is no consensus about it. Research papers refer to different low-pass filters, high-pass filters, baseline, subsampling or feature selection. In this paper, using a database with 23 healthy subjects we compare the effect on the letter accuracy (single-trial detection) provided by a linear support vector machine of a high-pass filter with cutoff frequencies from 0.1 to 1 Hz and a low-pass filter with cutoff frequencies from 8 to 60 Hz. According to this study, the best combination is for a band-pass filter of 0.1 to 15 Hz.