Depth variant image restoration in 3D fluorescence microscopy: two approaches under Gaussian and Poissonian noise conditions

Saima Ben Hadj 1 Laure Blanc-Féraud 1 Elie Maalouf 2 Bruno Colicchio 2 Alain Dieterlen 2
1 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, SIS - Signal, Images et Systèmes
Abstract : In this article, we are interested in restoring images from 3D fluorescence microscopy. In fact, these images are affected by a depth-variant blur due to light refraction phenomenon. We present and compare two different restoration strategies for that problem. The first one is based on multiple deconvolutions with depth-invariant blur functions and the second one consists in using a depth-variant blur function in the deconvolution process. Furthermore, we fit two deconvolution algorithms to this problem. First, we use the Richardson-Lucy method with total variation regularization to restore confocal microscopy images which are affected by a Poisson noise. Then, we focus on restoring wide field microscopy images which are corrupted by a Gaussian noise. Tests on simulated data show that the second restoration strategy is slightly more accurate than the first one for both noise conditions.
Type de document :
Communication dans un congrès
ISBI 2012 - 9th International Symposium on Biomedical Imaging, May 2012, Barcelona, Spain. IEEE, Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on, pp.1671-1674, 2012, 〈10.1109/ISBI.2012.6235899〉
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https://hal.inria.fr/hal-00765002
Contributeur : Laure Blanc-Féraud <>
Soumis le : jeudi 13 décembre 2012 - 22:51:40
Dernière modification le : jeudi 3 mai 2018 - 13:08:47

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Saima Ben Hadj, Laure Blanc-Féraud, Elie Maalouf, Bruno Colicchio, Alain Dieterlen. Depth variant image restoration in 3D fluorescence microscopy: two approaches under Gaussian and Poissonian noise conditions. ISBI 2012 - 9th International Symposium on Biomedical Imaging, May 2012, Barcelona, Spain. IEEE, Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on, pp.1671-1674, 2012, 〈10.1109/ISBI.2012.6235899〉. 〈hal-00765002〉

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