Role of RNA structure and protein factors in the control of HIV-1 splicing

Abstract : Alternative splicing plays a key role in the production of numerous proteins by complex lentiviruses such as HIV-1. The study of HIV-1 RNA splicing has provided useful information not only about the physiology of the virus, but also about the general mechanisms that regulate mammalian pre-mRNA alternative splicing. Like all retroviruses, a fraction of HIV-1 transcripts remains intact to serve as genomic RNA and to code for Gag and Gag-Pol protein precursors. In addition, splicing is important for controlling the production of some viral proteins, which could otherwise have a negative effect on the infected cell. Here, we summarize how the utilization of HIV-1 splicing sites is limited by the binding of nuclear factors to cis-acting silencer elements, taking into account the role of RNA secondary structure in these mechanisms. We also describe how the poorly efficient HIV-1 acceptor sites are nevertheless activated by serine/arginine-rich proteins. Finally, we discuss how nuclear factors that interact with both the transcription and splicing machineries also participate in the control of HIV-1 RNA splicing.
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https://hal.inria.fr/hal-00793011
Contributor : Gaëlle Rivérieux <>
Submitted on : Thursday, February 21, 2013 - 2:32:14 PM
Last modification on : Friday, June 28, 2019 - 9:38:04 AM

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Jean-Michel Saliou, Cyril Bourgeois, L. Ayadi-Ben Mena, Delphine Ropers, S. Jacquenet, et al.. Role of RNA structure and protein factors in the control of HIV-1 splicing. Frontiers in Bioscience, Frontiers in Bioscience, 2009, 14, pp.2714-2729. ⟨hal-00793011⟩

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