Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans.

Nicolas Seghezzi 1 Marie-Joelle Virolle 1 Patrick Amar 2, 3
3 AMIB - Algorithms and Models for Integrative Biology
CNRS - Centre National de la Recherche Scientifique : UMR8623, X - École polytechnique, Inria Saclay - Ile de France, UP11 - Université Paris-Sud - Paris 11, LRI - Laboratoire de Recherche en Informatique, LIX - Laboratoire d'informatique de l'École polytechnique [Palaiseau]
Abstract : ABSTRACT: A library of synthetic promoters of various strengths, specifically constructed for Streptomyces species, was cloned in the promoter-probe plasmid pIJ487, upstream of the promoter-less aphII gene that confers resistance to neomycin. The survival rates conferred by promoters were assessed in the presence of 100 mug.ml-1 neomycin. The correlation between the transcriptional activity of the aphII gene (estimated by RT-PCR) and the resistance to neomycin (expressed as survival rate) indicated a sigmoid rather than a linear correlation. In this issue, we propose a tentative explanation for this sigmoidal pattern of resistance in relation with the level of aphII gene expression. Beyond this specific example, our model might constitute a sound explanation for the generally observed but never explained sigmoidal shape of classical inhibition curves obtained in the presence of linearly increasing antibiotic concentrations.
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Article dans une revue
AMB Express, SpringerOpen, 2013, 3 (1), pp.13. 〈10.1186/2191-0855-3-13〉
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https://hal.inria.fr/hal-00794555
Contributeur : Patrick Amar <>
Soumis le : mardi 26 février 2013 - 10:29:02
Dernière modification le : jeudi 12 avril 2018 - 01:49:05

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Nicolas Seghezzi, Marie-Joelle Virolle, Patrick Amar. Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans.. AMB Express, SpringerOpen, 2013, 3 (1), pp.13. 〈10.1186/2191-0855-3-13〉. 〈hal-00794555〉

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