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Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans.

Nicolas Seghezzi 1 Marie-Joelle Virolle 1 Patrick Amar 2, 3 
3 AMIB - Algorithms and Models for Integrative Biology
LIX - Laboratoire d'informatique de l'École polytechnique [Palaiseau], LRI - Laboratoire de Recherche en Informatique, UP11 - Université Paris-Sud - Paris 11, Inria Saclay - Ile de France
Abstract : A library of synthetic promoters of various strengths, specifically constructed for Streptomyces species, was cloned in the promoter-probe plasmid pIJ487, upstream of the promoter-less aphII gene that confers resistance to neomycin. The survival rates conferred by promoters were assessed in the presence of 100 mug.ml-1 neomycin. The correlation between the transcriptional activity of the aphII gene (estimated by RT-PCR) and the resistance to neomycin (expressed as survival rate) indicated a sigmoid rather than a linear correlation. In this issue, we propose a tentative explanation for this sigmoidal pattern of resistance in relation with the level of aphII gene expression. Beyond this specific example, our model might constitute a sound explanation for the generally observed but never explained sigmoidal shape of classical inhibition curves obtained in the presence of linearly increasing antibiotic concentrations.
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https://hal.inria.fr/hal-00794555
Contributor : Patrick Amar Connect in order to contact the contributor
Submitted on : Tuesday, February 26, 2013 - 10:29:02 AM
Last modification on : Saturday, June 25, 2022 - 10:09:41 PM

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Nicolas Seghezzi, Marie-Joelle Virolle, Patrick Amar. Novel insights regarding the sigmoidal pattern of resistance to neomycin conferred by the aphII gene, in Streptomyces lividans.. AMB Express, 2013, 3 (1), pp.13. ⟨10.1186/2191-0855-3-13⟩. ⟨hal-00794555⟩

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