In most virtual reality (VR) simulations the virtual world is larger than the real walking workspace. The workspace is often bounded by the tracking area or the display devices. Hence, many researchers have proposed technical solutions to make people walk through large virtual spaces using various types of metaphors and multisensory feedback. To achieve this goal it is necessary to understand how people walk in real life. This chapter reports biomechanical data describing human walking including kinematics, dynamics and energetics knowledge for straight line and nonlinearwalking.Reference and normative values are provided formost of these variables, which could help developers and researchers improve the naturalness of walking in large virtual environments, or to propose evaluation metrics. For each section of this chapter, we will provide some potential applications in VR. On the one hand, this type of knowledge could be used to design more natural interaction devices such as omnidirectional treadmills,walk-in-placemethods, or other facilities. A specific section is dedicated to comparisons between treadmill and groundwalking as it is one of the most popular approaches in VR. On the other hand, this knowledge could also be useful to improve the quality of multisensory feedback when walking, such as adding sounds, vibrations, or more natural camera control.