A new variational method for erythrocyte velocity estimation in wide-field imaging in-vivo

Thomas Deneux 1, 2 Olivier Faugeras 3 Sylvain Takerkart 2 Guillaume Masson 2 Ivo Vanzetta 2
3 NEUROMATHCOMP
CRISAM - Inria Sophia Antipolis - Méditerranée , INRIA Rocquencourt, ENS Paris - École normale supérieure - Paris, UNS - Université Nice Sophia Antipolis, CNRS - Centre National de la Recherche Scientifique : UMR8548
Abstract : Measuring erythrocyte velocity in individual microvessels has important applications for biomedical and functional imaging. Recent multiphoton fluorescence microscopy approaches require injecting fluorescent tracers; moreover, only one or few vessels can be imaged at a time. To overcome these shortcomings, we used CCD-based optical imaging of intrinsic absorption changes in macroscopic vascular networks to record erythrocytes' trajectories over several mm2 of cortical surface. We then demonstrate the feasibility of erythrocyte velocity estimation from such wide-field data, using two robust, independent, algorithms. The first one is a recently published Radon transform-based algorithm that estimates erythrocyte velocity locally. We adapt it to data obtained in wide-field imaging and show, for the first time, its performance on such datasets. The second ("fasttrack") algorithm is novel. It is based on global energy minimization techniques to estimate the full spatiotemporal erythrocytes' trajectories inside vessels. We test the two algorithms on both simulated and biological data, obtained in rat cerebral cortex in a spreading depression experiment. On vessels with medium-slow erythrocyte velocities both algorithms performed well, allowing their usage as benchmark one for another. However, our novel fasttrack algorithm outperformed the other one for higher velocities, as encountered in the arterial network.
Type de document :
Article dans une revue
IEEE Transactions on Medical Imaging, Institute of Electrical and Electronics Engineers, 2011, 30 (8), pp.1527--1545. 〈10.1109/TMI.2011.2131151〉
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Soumis le : mercredi 17 juillet 2013 - 16:12:06
Dernière modification le : mardi 12 juin 2018 - 12:36:02

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Thomas Deneux, Olivier Faugeras, Sylvain Takerkart, Guillaume Masson, Ivo Vanzetta. A new variational method for erythrocyte velocity estimation in wide-field imaging in-vivo. IEEE Transactions on Medical Imaging, Institute of Electrical and Electronics Engineers, 2011, 30 (8), pp.1527--1545. 〈10.1109/TMI.2011.2131151〉. 〈hal-00845723〉

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