Global analysis of DNA methylation and transcription of human repetitive sequences. - Archive ouverte HAL Access content directly
Journal Articles Epigenetics Year : 2009

Global analysis of DNA methylation and transcription of human repetitive sequences.

(1) , (2) , (1) , (3) , (4) , (4) , (5) , (5) , (6) , (7, 8) , (1) , (9) , (1) , (2) , (1)
1
2
3
4
5
6
7
8
9
Gérard Roizes
Frédéric Devaux

Abstract

Half of the human genome consists of repetitive DNA sequences. Recent studies in various organisms highlight the role of chromatin regulation of repetitive DNA in gene regulation as well as in maintainance of chromosomes and genome integrity. Hence, repetitive DNA sequences might be potential "sensors" for chromatin changes associated with pathogenesis. Therefore, we developed a new genomic tool called RepArray. RepArray is a repeat-specific microarray composed of a representative set of human repeated sequences including transposon-derived repeats, simple sequences repeats, tandemly repeated sequences such as centromeres and telomeres. We showed that combined to anti-methylcytosine immunoprecipitation assay, the RepArray can be used to generate repeat-specific methylation maps. Using cell lines impaired chemically or genetically for DNA methyltransferases activities, we were able to distinguish different epigenomes demonstrating that repeats can be used as markers of genome-wide methylation changes. Besides, using a well-documented system model, the thermal stress, we demonstrated that RepArray is also a fast and reliable tool to obtain an overview of overall transcriptional activity on whole repetitive compartment in a given cell type. Thus, the RepArray represents the first valuable tool for systematic and genome-wide analyses of the methylation and transcriptional status of the repetitive counterpart of the human genome.
Fichier principal
Vignette du fichier
Global analysis of DNA methylation and transcription of human repetitive sequences.pdf (3.35 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-00851248 , version 1 (20-12-2017)

Identifiers

Cite

Béatrice Horard, Angéline Eymery, Geneviève Fourel, Nikita Vassetzky, Jacques Puechberty, et al.. Global analysis of DNA methylation and transcription of human repetitive sequences.. Epigenetics, 2009, 4 (5), pp.339-50. ⟨10.4161/epi.4.5.9284⟩. ⟨hal-00851248⟩
741 View
325 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More