Hyperosmotic stress contributes to mouse colonic inflammation through the methylation of protein phosphatase 2A.

Laurent Schwartz 1 Mohammad Abolhassani 2 Mohammad Pooya 2 Jean-Marc Steyaert 1, 3 Xavier Wertz 1, 4 Maurice Israël 5 Adeline Guais 6 Philippe Chaumet-Riffaud 7
3 AMIB - Algorithms and Models for Integrative Biology
LIX - Laboratoire d'informatique de l'École polytechnique [Palaiseau], LRI - Laboratoire de Recherche en Informatique, UP11 - Université Paris-Sud - Paris 11, Inria Saclay - Ile de France, X - École polytechnique, CNRS - Centre National de la Recherche Scientifique : UMR8623
Abstract : There are several reports suggesting hyperosmotic contents in the feces of patients suffering from inflammatory bowel disease (IBD). Previous works have documented that hyperosmolarity can cause inflammation attributable to methylation of the catalytic subunit of protein phosphatase 2A (PP2A) and subsequent NF-kappaB activation resulting in cytokine secretion. In this study, we demonstrate that dextran sulfate sodium (DSS) induces colitis due to hyperosmolarity and subsequent PP2A activation. Mice were randomized and fed with increased concentrations of DSS (0 mOsm, 175 mOsm, 300 mOsm, and 627 mOsm) for a duration of 3 wk or with hyperosmotic concentrations of DSS (627 mOsm) or mannitol (450 mOsm) for a duration of 12 wk. Long-term oral administration of hyposmotic DSS or mannitol had no demonstrable effect. Hyperosmotic DSS or mannitol produced a significant increase in colonic inflammation, as well as an increase in the weight of sacral lymph nodes and in serum amyloid A protein levels. Similar results were obtained through the ingestion of comparable osmolarities of mannitol. Hyperosmolarity induces the methylation of PP2A, nuclear p65 NF-kappaB activation. and cytokine secretion. The rectal instillation of okadaic acid, a well-known PP2A inhibitor, reverses the IBD. Short inhibiting RNAs (siRNAs) targeted toward PP2Ac reverse the effect of hyperosmotic DSS. The present study strongly suggests that DSS-induced chronic colitis is a consequence of the methylation of PP2Ac induced by hyperosmolarity.
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AJP - Gastrointestinal and Liver Physiology, American Physiological Society, 2008, 295 (5), pp.G934-41. 〈10.1152/ajpgi.90296.2008〉
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Soumis le : vendredi 6 septembre 2013 - 12:30:23
Dernière modification le : jeudi 10 mai 2018 - 02:06:01

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Laurent Schwartz, Mohammad Abolhassani, Mohammad Pooya, Jean-Marc Steyaert, Xavier Wertz, et al.. Hyperosmotic stress contributes to mouse colonic inflammation through the methylation of protein phosphatase 2A.. AJP - Gastrointestinal and Liver Physiology, American Physiological Society, 2008, 295 (5), pp.G934-41. 〈10.1152/ajpgi.90296.2008〉. 〈hal-00858998〉

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