Head Tracking and Flagellum Tracing for Sperm Motility Analysis

Huei-Fang Yang 1 Xavier Descombes 1 Sylvain Prigent 1 Grégoire Malandain 1 Xavier Druart Franck Plouraboué 2
1 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, Laboratoire I3S - SIS - Signal, Images et Systèmes
Abstract : Sperm quality assessment plays an essential role in human fertility and animal breeding. Manual analysis is time-consuming and subject to intra- and inter-observer variability. To automate the analysis process, as well as to offer a means of statistical analysis that may not be achieved by visual inspection, we present a computational framework that tracks the heads and traces the tails for analyzing sperm motility, one of the most important attributes in semen quality evaluation. Our framework consists of 3 modules: head detection, head tracking, and flagellum tracing. The head detection module detects the sperm heads from the image data, and the detected heads are the inputs to the head tracking module for obtaining the head trajectories. Finally, a flagellum tracing algorithm is proposed to obtain the flagellar beat patterns. Our framework aims at providing both the head trajectories and the flagellar beat patterns for quantitatively assessing sperm motility. This distinguishes our work from other existing methods that analyze sperm motility based merely on the head trajectories. We validate our framework using two confocal microscopy image sequences of ram semen samples that were imaged at two different conditions, at which the sperms behave differently. The results show the effectiveness of our framework.
Document type :
Conference papers
Liste complète des métadonnées

Cited literature [9 references]  Display  Hide  Download

https://hal.inria.fr/hal-00909868
Contributor : Huei-Fang Yang <>
Submitted on : Friday, December 13, 2013 - 8:40:22 AM
Last modification on : Friday, April 12, 2019 - 4:22:39 PM
Document(s) archivé(s) le : Friday, March 14, 2014 - 1:10:09 AM

File

isbi14.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Huei-Fang Yang, Xavier Descombes, Sylvain Prigent, Grégoire Malandain, Xavier Druart, et al.. Head Tracking and Flagellum Tracing for Sperm Motility Analysis. ISBI - International Symposium on Biomedical Imaging, Apr 2014, Beijing, China. ⟨10.1109/ISBI.2014.6867871⟩. ⟨hal-00909868v2⟩

Share

Metrics

Record views

864

Files downloads

609