A 3D model with shape prior information for biological structures reconstruction using Multiple-Angle Total Internal Reflection Fluorescence Microscopy

Emmanuel Soubies 1 Laure Blanc-Féraud 1 Sébastien Schaub 2 Gilles Aubert 3
1 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, Laboratoire I3S - SIS - Signal, Images et Systèmes
Abstract : We propose a new model for the reconstruction of biological struc- tures using Multiple-Angle Total Internal Reflection Fluorescence Microscopy (MA-TIRFM). This recent microscopy technique allows the visualization of sub-cellular structures around the plasma mem- brane which is of fundamental importance in the comprehension of exchanges mechanisms of the cell. We present a 3D reconstruction method based on a shape prior information on the observed struc- tures and robust to shot noise and background fluorescence. A nov- elty with respect to the state of the art is to propose a method allow- ing the recovery of multiple objects aligned along the axial axis. The optimization problem can be formulated as a minimization problem where both the number of objects in the model and their parame- ters have to be estimated. This difficult combinatorial optimization problem is tackled by using a Marked Point Process approach which allows modelling interactions between the objects in order to regu- larize the inverse problem. Finally, performances of the proposed method are evaluated on synthetic data and real data.
Complete list of metadatas

Cited literature [11 references]  Display  Hide  Download

https://hal.inria.fr/hal-00914998
Contributor : Emmanuel Soubies <>
Submitted on : Friday, December 6, 2013 - 1:35:05 PM
Last modification on : Monday, November 5, 2018 - 3:52:02 PM
Long-term archiving on : Saturday, April 8, 2017 - 5:06:21 AM

File

Soubies_ISBI_2014.pdf
Files produced by the author(s)

Identifiers

Citation

Emmanuel Soubies, Laure Blanc-Féraud, Sébastien Schaub, Gilles Aubert. A 3D model with shape prior information for biological structures reconstruction using Multiple-Angle Total Internal Reflection Fluorescence Microscopy. ISBI - International Symposium on Biomedical Imaging, Apr 2014, Beijing, China. pp.608 - 611, ⟨10.1109/ISBI.2014.6867944⟩. ⟨hal-00914998⟩

Share

Metrics

Record views

1217

Files downloads

456