Rapid, modular and reliable construction of complex mammalian gene circuits

Abstract : We developed a framework for quick and reliable construction of complex gene circuits for genetically engineering mammalian cells. Our hierarchical framework is based on a novel nucleotide addressing system for defining the position of each part in an overall circuit. With this framework, we demonstrate construction of synthetic gene circuits of up to 64 kb in size comprising 11 transcription units and 33 basic parts. We show robust gene expression control of multiple transcription units by small molecule inducers in human cells with transient transfection and stable chromosomal integration of these circuits. This framework enables development of complex gene circuits for engineering mammalian cells with unprecedented speed, reliability and scalability and should have broad applicability in a variety of areas including mammalian cell fermentation, cell fate reprogramming and cell-based assays.
Type de document :
Article dans une revue
Nucleic Acids Research, Oxford University Press, 2013, 41 (16), pp.e156. 〈http://nar.oxfordjournals.org/content/41/16/e156.full〉. 〈10.1093/nar/gkt605〉
Liste complète des métadonnées

Littérature citée [26 références]  Voir  Masquer  Télécharger

https://hal.inria.fr/hal-00926598
Contributeur : Gregory Batt <>
Soumis le : mercredi 15 janvier 2014 - 01:24:05
Dernière modification le : jeudi 14 décembre 2017 - 14:12:02
Document(s) archivé(s) le : mardi 15 avril 2014 - 22:04:13

Fichier

Nucl._Acids_Res.-2013-Guye-e15...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Patrick Guye, Yinqing Li, Liliana Wroblewska, Xavier Duportet, Ron Weiss. Rapid, modular and reliable construction of complex mammalian gene circuits. Nucleic Acids Research, Oxford University Press, 2013, 41 (16), pp.e156. 〈http://nar.oxfordjournals.org/content/41/16/e156.full〉. 〈10.1093/nar/gkt605〉. 〈hal-00926598〉

Partager

Métriques

Consultations de la notice

160

Téléchargements de fichiers

288