Robust parametric stabilization of moving cells with intensity correction in light microscopy image sequences

Solene Ozere 1 Patrick Bouthemy 1 Fabien Spindler 2 Perrine Paul-Gilloteaux 3, 4 Charles Kervrann 1
2 Lagadic - Visual servoing in robotics, computer vision, and augmented reality
CRISAM - Inria Sophia Antipolis - Méditerranée , Inria Rennes – Bretagne Atlantique , IRISA-D5 - SIGNAUX ET IMAGES NUMÉRIQUES, ROBOTIQUE
3 Mécanismes moléculaires du transport intracellulaire
CDC - Compartimentation et dynamique cellulaires
Abstract : Automatically stabilizing moving living cells in fluorescence microscopy image sequences is required to attain and analyze the actual displacements of subcellular particles. We have designed a stabilization method which can handle within a single parametric framework, the estimation of the global motion and of the temporal intensity variation (e.g., due to photobleaching effect) that we have to compensate for. We have introduced extended parametric motion-intensity constraints and exploited a robust multiresolution estimation scheme insensitive to local independent motions (outliers). We demonstrate the efficiency and the accuracy of our stabilization method on three challenging cellular events: cell development, endosome displacements, protein recruitment.
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Conference papers
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https://hal.inria.fr/hal-00931371
Contributor : Charles Kervrann <>
Submitted on : Wednesday, January 15, 2014 - 11:54:02 AM
Last modification on : Monday, August 19, 2019 - 3:32:04 PM

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Solene Ozere, Patrick Bouthemy, Fabien Spindler, Perrine Paul-Gilloteaux, Charles Kervrann. Robust parametric stabilization of moving cells with intensity correction in light microscopy image sequences. ISBI - IEEE International Symposium on Biomedical Imaging, Apr 2013, San-Francisco, United States. pp.468-471, ⟨10.1109/ISBI.2013.6556513⟩. ⟨hal-00931371⟩

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