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Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.

Abstract : Drosophila melanogaster is a model organism rich in genetic tools to manipulate and identify neural circuits involved in specific behaviors. Here we present a technique for two-photon calcium imaging in the central brain of head-fixed Drosophila walking on an air-supported ball. The ball's motion is tracked at high resolution and can be treated as a proxy for the fly's own movements. We used the genetically encoded calcium sensor, GCaMP3.0, to record from important elements of the motion-processing pathway, the horizontal-system lobula plate tangential cells (LPTCs) in the fly optic lobe. We presented motion stimuli to the tethered fly and found that calcium transients in horizontal-system neurons correlated with robust optomotor behavior during walking. Our technique allows both behavior and physiology in identified neurons to be monitored in a genetic model organism with an extensive repertoire of walking behaviors.
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https://hal.inria.fr/hal-01062437
Contributor : Anirban Dutta <>
Submitted on : Tuesday, September 9, 2014 - 5:33:48 PM
Last modification on : Thursday, February 25, 2021 - 7:04:11 PM

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Johannes D Seelig, M Eugenia Chiappe, Gus K Lott, Anirban Dutta, Jason E Osborne, et al.. Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.. Nature Methods, Nature Publishing Group, 2010, 7 (7), pp.535-40. ⟨10.1038/nmeth.1468⟩. ⟨hal-01062437⟩

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