The effect of retinal GABA Depletion by Allylglycine on mouse retinal ganglion cell responses to light

Gerrit Hilgen 1 Samantha Softley 1 Daniela Pamplona 2 Pierre Kornprobst 2 Bruno Cessac 2 Evelyne Sernagor 1
2 NEUROMATHCOMP - Mathematical and Computational Neuroscience
CRISAM - Inria Sophia Antipolis - Méditerranée , JAD - Laboratoire Jean Alexandre Dieudonné : UMR6621
Abstract : The inhibitory neurotransmitter GABA (γ-aminobutyric acid) is metabolized by glutamic acid decarboxylase (GAD) which exists in two iso- forms in the mature CNS, GAD65 and GAD67. Allylglycine, a glycine derivative, is a nonspecific inhibitor of both GAD isoforms. Prolonged exposure to allylgycine can therefore deplete the tissue of endogenous GABA over time (Orlowski et al, 1977; Chabrol et al., 2012). Here we applied Allylglycine (ALLYL) in vitro over several hours to gradually deplete GABA in the adult mouse retina and compared the effects of GABA depletion on retinal ganglion cells (RGCs) receptive fields with those obtained by simultaneously blocking all GABAergic recep- tors (type A, B and C).
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Submitted on : Monday, November 30, 2015 - 8:35:53 AM
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Gerrit Hilgen, Samantha Softley, Daniela Pamplona, Pierre Kornprobst, Bruno Cessac, et al.. The effect of retinal GABA Depletion by Allylglycine on mouse retinal ganglion cell responses to light . European Retina Meeting, Oct 2015, Brigthon, United Kingdom. ⟨hal-01235324⟩

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