Towards structural models for the Ebola UTR regions using experimental SHAPE probing data - Archive ouverte HAL Access content directly
Conference Papers Year : 2016

Towards structural models for the Ebola UTR regions using experimental SHAPE probing data

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Abstract

Next-Generation Sequencing (NGS) technologies have opened new perspectives to refine the process of predicting the secondary structure(s) of structured non-coding RNAs. Herein, we describe an integrated modeling strategy, based on the SHAPE chemistry, to infer structural insight from deep se-quencing data. Our approach is based on a pseudo-energy minimization, incorporating additional information from evolutionary data (compensatory mutations) and SHAPE experiments (reactivity scores) within an iterative procedure. Preliminary results reveal conserved and stable structures within UTRs of the Ebola Genome, that are both thermodynamically-stable and highly supported by SHAPE accessibility analysis.
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Dates and versions

hal-01332469 , version 1 (15-06-2016)

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Attribution - CC BY 4.0

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  • HAL Id : hal-01332469 , version 1

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Afaf Saaidi, Delphine Allouche, Bruno Sargueil, Yann Ponty. Towards structural models for the Ebola UTR regions using experimental SHAPE probing data. JOBIM - Journées Ouvertes en Biologie, Informatique et Mathématiques - 2016, Jun 2016, Lyon, France. ⟨hal-01332469⟩
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