Abstract : Does the stimulation used during the training on an SSVEP-based BCI have to be similar to that of the end use? We recorded six-channel EEG data from 12 subjects in various conditions of distance between targets, and of difference in color between targets. Our analysis revealed that the stimulation configuration used for training which leads to the best classification accuracy is not always the one which is closest to the end use configuration. We found that the distance between targets during training is of little influence if the end use targets are close to each other, but that training at far distance can lead to a better accuracy for far distance end use (p < .01). Additionally, an interaction effect is observed between training and testing color (p < .001): while training with monochrome targets leads to good performance only when the test context involves monochrome targets as well, a classifier trained on colored targets can be efficient for both colored and monochrome targets. In a nutshell, in the context of SSVEP-based BCI, training using distant targets of different colors seems to lead to the best and more robust performance in all end use contexts.