Nowadays, virtual organizations (VOs) need to utilize new technologies in order to respond rapidly to collaboration opportunities. Cloud computing is a new paradigm that could leverage VO efficiency and flexibility whilst it could decrease VO expenditures and response time. Also, VO breeding environment (VBE) is an approach to create efficient VOs. Thus, combination of VBE and cloud computing may facilitate VOs creation and reactions to upcoming opportunities. This paper has proposed a framework to create VO on cloud environment, including three phases: creating and managing VBE on cloud, moving VBE on cloud and finally creating VO on cloud. The proposed framework may be considered as a solution to cope with VO requirements.