The pulling of the glass microelectrode is a necessary technology in the electrophysiological measurement techniques. In order to better meet the requirements of electrophysiological experiments, we designed a multi-functional microelectrode puller, and gave the design of functional modules, and discussed its controlling effect. In this paper, we analyze the impact on the glass microelectrodes pulling which is brought by the heating strength based on the correspondence between the heating temperature and the heating wire resistance. Experimental results showed that the direct measurement of the heating temperature during the glass microelectrodes pulling process provided more direct control basis for the intelligent control, and provided a subtle improvement ideas for achieving precise pulling of the pipette puller.