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Accelerated gradient-based methods for phase estimation in differential-interference-contrast microscopy

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Abstract

In the last forty years, differential-interference-contrast (DIC) microscopy has gained popularity in biomedical research as an effective optical microscopy technique used to observe unstained transparent specimens under a transmitted-light configuration. The DIC image formation is caused by the interference of two orthogonally polarized beams, which are laterally split of a few tenths of a micrometer by a Wollaston prism, phase shifted when passing through different materials across the specimen and then successively recombined by a sliding prism. The resulting image has a three dimensional, high contrast appearance, which can be enhanced by adjusting the sliding prism along the direction of the split in order to introduce a uniform phase difference between the two beams.
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Dates and versions

hal-01426330 , version 1 (04-01-2017)

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  • HAL Id : hal-01426330 , version 1

Cite

Simone Rebegoldi, Lola Bautista, Laure Blanc-Féraud, Marco Prato, Luca Zanni, et al.. Accelerated gradient-based methods for phase estimation in differential-interference-contrast microscopy. SIMAI 2016: Società Italiana di Matematica Aplicata e Industriale, Sep 2016, Milan, Italy. ⟨hal-01426330⟩
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