Skip to Main content Skip to Navigation
New interface
Preprints, Working Papers, ...

3D ConvNet improves macromolecule localization in 3D cellular cryo-electron tomograms

Abstract : Cryo-electron tomography (cryo-ET) allows one to capture 3D images of cells in a close to native state, at sub-nanometer resolution. However, noise and artifact levels are such that heavy computational processing is needed to access the image content. In this paper, we propose a deep learning framework to accurately and jointly localize multiple types and states of macromolecules in cellular cryo-electron tomograms. We compare this framework to the commonly-used template matching method on both synthetic and experimental data. On synthetic image data, we show that our framework is very fast and produces superior detection results. On experimental data, the detection results obtained by our method correspond to an overlap rate of 86% with the expert annotations, and comparable resolution is achieved when applying subtomogram averaging. In addition, we show that our method can be combined to template matching procedures to reliably increase the number of expected detections. In our experiments, this strategy was able to find additional 20.5% membrane-bound ribosomes that were missed or discarded during manual annotation.
Complete list of metadata

Cited literature [20 references]  Display  Hide  Download
Contributor : Charles Kervrann Connect in order to contact the contributor
Submitted on : Saturday, December 29, 2018 - 7:07:07 PM
Last modification on : Thursday, September 1, 2022 - 9:02:10 AM
Long-term archiving on: : Saturday, March 30, 2019 - 2:11:07 PM


Files produced by the author(s)


  • HAL Id : hal-01966819, version 1


Emmanuel Moebel, Antonio Martinez, Damien Larivière, Julio Ortiz, Wolfgang Baumeister, et al.. 3D ConvNet improves macromolecule localization in 3D cellular cryo-electron tomograms. 2018. ⟨hal-01966819⟩



Record views


Files downloads