HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Numerical Optimization of Plasmid DNA Delivery Combined with Hyaluronidase Injection for Electroporation Protocol

Daniele Peri 1 Manon Deville 2 Clair Poignard 2 Emanuela Signori 3 Roberto Natalini 1
1 IAC - Istituto per le Applicazioni del Calcolo "Mauro Picone"
2 MONC - Modélisation Mathématique pour l'Oncologie
IMB - Institut de Mathématiques de Bordeaux, Institut Bergonié [Bordeaux], Inria Bordeaux - Sud-Ouest
3 CNR - Consiglio Nazionale delle Ricerche [Roma]
Abstract : Background and Objective: The paper focuses on the numerical strategies to optimize a plasmid DNA delivery protocol, which combines hyaluronidase and electroporation. Methods: A well-defined continuum mechanics model of muscle porosity and advanced numerical optimization strategies have been used, to propose a substantial improvement of a pre-existing experimental protocol of DNA transfer in mice. Our work suggests that a computational model might help in the definition of innovative therapeutic procedures, thanks to the fine tuning of all the involved experimental steps. This approach is particularly interesting in optimizing complex and costly protocols, to make in vivo DNA therapeutic protocols more effective. Results: Our preliminary work suggests that computational model might help in the definition of innovative therapeutic protocol, thanks to the fine tuning of all the involved operations. Conclusions: This approach is particularly interesting in optimizing complex and costly protocols for which the number of degrees of freedom prevents a experimental test of the possible configuration.
Document type :
Journal articles
Complete list of metadata

Cited literature [44 references]  Display  Hide  Download
https://hal.inria.fr/hal-02366660
Contributor : Clair Poignard Connect in order to contact the contributor
Submitted on : Monday, November 18, 2019 - 8:52:19 AM
Last modification on : Wednesday, February 2, 2022 - 3:54:41 PM

Annex

Identifiers

Collections

INSMI | BERGONIE | INRIA2 | INRIA | CNRS | TDS-MACS

Citation

Daniele Peri, Manon Deville, Clair Poignard, Emanuela Signori, Roberto Natalini. Numerical Optimization of Plasmid DNA Delivery Combined with Hyaluronidase Injection for Electroporation Protocol. Computer Methods and Programs in Biomedicine, Elsevier, 2019, pp.105204. ⟨10.1016/j.cmpb.2019.105204⟩. ⟨hal-02366660⟩

Export

BibTeX TEI DC DCterms EndNote Datacite

Share

Metrics

Record views

19

Files downloads

31

 

Contact                    Legal Notice                    Personal Data