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Master thesis

Vérifications du réseau métabolique entier de Tisochrysis lutea

Nicolas Guillaudeux 1
1 Dyliss - Dynamics, Logics and Inference for biological Systems and Sequences
Inria Rennes – Bretagne Atlantique , IRISA-D7 - GESTION DES DONNÉES ET DE LA CONNAISSANCE
Abstract : Metabolic network reconstruction is a new critical step to connect massive sequencing and biochemical data with metabolic information contained in databases. In this context, the aim of this internship was to reconstruct the metabolic network of the Tisochrysis lutea (T. lutea) microalgae through a workflow developed by the Dyliss team. This workflow combines several methods such as genomic annotation based reconstruction and orthology based reconstruction. It allows the gap-filling and manual curation as well as the analysis of the flux of these networks. The metabolic network reconstruction of T. lutea was carried out on one hand, from the combination of two distinct annotations, one obtained experimentally and the other in silico, on the other hand from the orthology informations of four organisms (Arabidopsis thaliana, Chlamydomonas reinhardtii, Ectocarpus siliculosus et Synechocystis sp. PCC 6803). The metabolic network obtained grows on its growth medium. Following this, a verification of this network was carried out on the analysis of a specific biosynthetic pathway: the carnosine biosynthesis pathway. The resulting figure is part of an article in preparation which co-authors I figure among.
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Master thesis
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Submitted on : Wednesday, November 27, 2019 - 2:34:24 PM
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Nicolas Guillaudeux. Vérifications du réseau métabolique entier de Tisochrysis lutea. Informatique [cs]. 2017. ⟨hal-02382948⟩

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