This book constitutes the proceedings of the 16th International Conference on Detection of Intrusions and Malware, and Vulnerability Assessment, DIMVA 2019, held in Gothenburg, Sweden, in June 2019. The 23 full papers presented in this volume were carefully reviewed and selected from 80 submissions. The contributions were organized in topical sections named: wild wild web; cyber-physical systems; malware; software security and binary analysis; network security; and attack mitigation.