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Contact area-dependent cell communication and the morphological invariance of ascidian embryogenesis

Léo Guignard 1, 2 Ulla-Maj Fiuza 2 Bruno Leggio 3 Julien Laussu 2 Emmanuel Faure 4 Gaël Michelin 5 Kilian Biasuz 2 Lars Hufnagel 6 Grégoire Malandain 5 Christophe Godin 7, 1 Patrick Lemaire 8
1 VIRTUAL PLANTS - Modeling plant morphogenesis at different scales, from genes to phenotype
CRISAM - Inria Sophia Antipolis - Méditerranée , INRA - Institut National de la Recherche Agronomique, UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales
4 IRIT-REVA - Real Expression Artificial Life
IRIT - Institut de recherche en informatique de Toulouse
5 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, Laboratoire I3S - SIS - Signal, Images et Systèmes
Abstract : Marine invertebrate ascidians display embryonic reproducibility: Their early embryonic cell lineages are considered invariant and are conserved between distantly related species, despite rapid genomic divergence. Here, we address the drivers of this reproducibility. We used light-sheet imaging and automated cell segmentation and tracking procedures to systematically quantify the behavior of individual cells every 2 minutes during Phallusia mammillata embryogenesis. Interindividual reproducibility was observed down to the area of individual cell contacts. We found tight links between the reproducibility of embryonic geometries and asymmetric cell divisions, controlled by differential sister cell inductions. We combined modeling and experimental manipulations to show that the area of contact between signaling and responding cells is a key determinant of cell communication. Our work establishes the geometric control of embryonic inductions as an alternative to classical morphogen gradients and suggests that the range of cell signaling sets the scale at which embryonic reproducibility is observed.
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https://hal.inria.fr/hal-02903409
Contributor : Christophe Godin <>
Submitted on : Tuesday, July 21, 2020 - 9:45:47 AM
Last modification on : Thursday, July 23, 2020 - 11:22:39 AM

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Léo Guignard, Ulla-Maj Fiuza, Bruno Leggio, Julien Laussu, Emmanuel Faure, et al.. Contact area-dependent cell communication and the morphological invariance of ascidian embryogenesis. Science, American Association for the Advancement of Science, 2020, ⟨10.1126/science.aar5663⟩. ⟨hal-02903409⟩

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