Skip to Main content Skip to Navigation
Journal articles

Mycoplasma hyopneumoniae J elicits an antioxidant response and decreases the expression of ciliary genes in infected swine epithelial cells

Abstract : Mycoplasma hyopneumoniae is the most costly pathogen for swine production. Although several studies have focused on the host-bacterium association, little is known about the changes in gene expression of swine cells upon infection. To improve our understanding of this interaction, we infected swine epithelial nptr cells with M. hyopneumoniae strain J to identify differentially expressed mRNAs and miRNAs. The levels of 1,268 genes and 170 miRNAs were significantly modified post-infection. Up-regulated mRNAs were enriched in genes related to redox homeostasis and antioxidant defense, known to be regulated by the transcription factor NRF2 in related species. Down-regulated mRNAs were enriched in genes associated with cytoskeleton and ciliary functions. Bioinformatic analyses suggested a correlation between changes in miRNA and mRNA levels, since we detected down-regulation of miRNAs predicted to target antioxidant genes and up-regulation of miRNAs targeting ciliary and cytoskeleton genes. Interestingly, most down-regulated miRNAs were detected in exosome-like vesicles suggesting that M. hyopneumoniae infection induced a modification of the composition of NPTr-released vesicles. Taken together, our data indicate that M. hyopneumoniae elicits an antioxidant response induced by NRF2 in infected cells. In addition, we propose that ciliostasis caused by this pathogen is partially explained by the down-regulation of ciliary genes.
Document type :
Journal articles
Complete list of metadata

Cited literature [141 references]  Display  Hide  Download

https://hal.inria.fr/hal-02916844
Contributor : Marie-France Sagot <>
Submitted on : Tuesday, August 18, 2020 - 9:43:04 AM
Last modification on : Tuesday, September 14, 2021 - 2:46:02 PM
Long-term archiving on: : Tuesday, December 1, 2020 - 5:17:38 PM

File

s41598-020-70040-y.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Scheila Mucha, Mariana Ferrarini, Carol Moraga, Alex Genova, Laurent Guyon, et al.. Mycoplasma hyopneumoniae J elicits an antioxidant response and decreases the expression of ciliary genes in infected swine epithelial cells. Scientific Reports, Nature Publishing Group, 2020, 10 (13707), ⟨10.1038/s41598-020-70040-y⟩. ⟨hal-02916844⟩

Share

Metrics

Record views

137

Files downloads

575