Skip to Main content Skip to Navigation
Journal articles

A bacterial size law revealed by a coarse-grained model of cell physiology

Abstract : Universal observations in Biology are sometimes described as “laws”. In E. coli, experimental studies performed over the past six decades have revealed major growth laws relating ribosomal mass fraction and cell size to the growth rate. Because they formalize complex emerging principles in biology, growth laws have been instrumental in shaping our understanding of bacterial physiology. Here, we discovered a novel size law that connects cell size to the inverse of the metabolic proteome mass fraction and the active fraction of ribosomes. We used a simple whole-cell coarse-grained model of cell physiology that combines the proteome allocation theory and the structural model of cell division. This integrated model captures all available experimental data connecting the cell proteome composition, ribosome activity, division size and growth rate in response to nutrient quality, antibiotic treatment and increased protein burden. Finally, a stochastic extension of the model explains non-trivial correlations observed in single cell experiments including the adder principle. This work provides a simple and robust theoretical framework for studying the fundamental principles of cell size determination in unicellular organisms.
Document type :
Journal articles
Complete list of metadata

https://hal.inria.fr/hal-03152985
Contributor : Gregory Batt <>
Submitted on : Thursday, March 18, 2021 - 4:24:41 PM
Last modification on : Friday, March 19, 2021 - 9:30:51 AM

File

pcbi.1008245.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

François Bertaux, Julius von Kügelgen, Samuel Marguerat, Vahid Shahrezaei. A bacterial size law revealed by a coarse-grained model of cell physiology. PLoS Computational Biology, Public Library of Science, 2020, 16 (9), ⟨10.1371/journal.pcbi.1008245⟩. ⟨hal-03152985⟩

Share

Metrics

Record views

89

Files downloads

191