Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

Romain Marlin; Veronique Godot; Sylvain Cardinaud; Mathilde Galhaut; Severin Coleon; Sandra Zurawski; Nathalie Dereuddre-Bosquet; Mariangela Cavarelli; Anne-Sophie Gallouët; Pauline Maisonnasse; Léa Dupaty; Craig Fenwick; Thibaut Naninck; Julien Lemaitre; Mario Gomez-Pacheco; Nidhal Kahlaoui; Vanessa Contreras; Francis Relouzat; Raphaël Ho Tsong Fang; Zhiqing Wang; Jerome Ellis; Catherine Chapon; Mireille Centlivre; Aurelie Wiedemann; Christine Lacabaratz; Mathieu Surenaud; Inga Szurgot; Peter Liljeström; Delphine Planas; Timothée Bruel; Olivier Schwartz; Sylvie van Der Werf; Giuseppe Pantaleo; Mélanie Prague; Rodolphe Thiébaut; Gerard Zurawski; Yves Lévy; Roger Le Grand

doi:10.1038/s41467-021-25382-0

Journal Articles Nature Communications Year : 2021

Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

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Romain Marlin

Function : Author
PersonId : 778115
ORCID : 0000-0001-8932-0171

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Veronique Godot

Function : Author

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Sylvain Cardinaud

Function : Author
PersonId : 762692
ORCID : 0000-0002-2650-1108
IdRef : 259870595

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Mathilde Galhaut

Function : Author
PersonId : 818370
ORCID : 0000-0002-2607-4855

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Severin Coleon

Function : Author

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Sandra Zurawski

Function : Author
PersonId : 765664
ORCID : 0000-0002-8867-3765

Institut Mondor de Recherche Biomédicale

Baylor Scott & White Charles A. Sammons Cancer Center [Dallas, TX, USA]

Vaccine Research Institute

Nathalie Dereuddre-Bosquet

Function : Author
PersonId : 765411
ORCID : 0000-0001-6682-6313

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Mariangela Cavarelli

Function : Author
PersonId : 818371
ORCID : 0000-0002-8468-0263

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Anne-Sophie Gallouët

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Pauline Maisonnasse

Function : Author
PersonId : 774867
ORCID : 0000-0002-0555-207X
IdRef : 167097105

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Léa Dupaty

Function : Author

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Craig Fenwick

Function : Author

Lausanne University Hospital

Université de Lausanne = University of Lausanne

Thibaut Naninck

Function : Author
PersonId : 801959
ORCID : 0000-0002-6261-6412

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Julien Lemaitre

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Mario Gomez-Pacheco

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Nidhal Kahlaoui

Function : Author
PersonId : 801960
ORCID : 0000-0003-4784-0819

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Vanessa Contreras

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Francis Relouzat

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Raphaël Ho Tsong Fang

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Zhiqing Wang

Function : Author

Institut Mondor de Recherche Biomédicale

Baylor Scott & White Charles A. Sammons Cancer Center [Dallas, TX, USA]

Vaccine Research Institute

Jerome Ellis

Function : Author
PersonId : 818372
ORCID : 0000-0001-5224-3889

Institut Mondor de Recherche Biomédicale

Baylor Scott & White Charles A. Sammons Cancer Center [Dallas, TX, USA]

Vaccine Research Institute

Catherine Chapon

Function : Author

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Mireille Centlivre

Function : Author
PersonId : 755800
ORCID : 0000-0001-7616-9775

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Aurelie Wiedemann

Function : Author
PersonId : 759951
ORCID : 0000-0002-4224-3137

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Christine Lacabaratz

Function : Author
PersonId : 757905
ORCID : 0000-0001-6925-0225

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Mathieu Surenaud

Function : Author
PersonId : 817564
ORCID : 0000-0002-3312-7871

Institut Mondor de Recherche Biomédicale

Vaccine Research Institute

Inga Szurgot

Function : Author

Karolinska Institutet [Stockholm]

Peter Liljeström

Function : Author

Karolinska Institutet [Stockholm]

Delphine Planas

Function : Author
PersonId : 805693
ORCID : 0000-0002-2509-9954

Virus et Immunité - Virus and immunity

Vaccine Research Institute

Timothée Bruel

Function : Author
PersonId : 746191
IdHAL : timothee-bruel
ORCID : 0000-0002-3952-4261
IdRef : 169340171

Virus et Immunité - Virus and immunity

Vaccine Research Institute

Olivier Schwartz

Function : Author
PersonId : 757442
ORCID : 0000-0002-0729-1475
IdRef : 128673850

Virus et Immunité - Virus and immunity

Vaccine Research Institute

Sylvie van Der Werf

Function : Author
PersonId : 758475
ORCID : 0000-0002-1148-4456
IdRef : 070954534

Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses

Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris]

Giuseppe Pantaleo

Function : Author
PersonId : 758701
ORCID : 0000-0003-3651-2721

Vaccine Research Institute

Lausanne University Hospital

Université de Lausanne = University of Lausanne

Mélanie Prague

Function : Author
PersonId : 11146
IdHAL : mprague
ORCID : 0000-0001-9809-7848
IdRef : 175063427

Vaccine Research Institute

Bordeaux population health

Statistics In System biology and Translational Medicine

CHU Bordeaux [Bordeaux]

Rodolphe Thiébaut

Function : Author
PersonId : 175125
IdHAL : rodolphe-thiebaut
ORCID : 0000-0002-5235-3962
IdRef : 071580735

Statistics In System biology and Translational Medicine

Vaccine Research Institute

Bordeaux population health

CHU Bordeaux [Bordeaux]

Gerard Zurawski

Function : Author
PersonId : 765623
ORCID : 0000-0002-0168-3523

Vaccine Research Institute

Institut Mondor de Recherche Biomédicale

Baylor Scott & White Charles A. Sammons Cancer Center [Dallas, TX, USA]

Yves Lévy

Function : Correspondent author
PersonId : 929996

Connectez-vous pour contacter l'auteur

Vaccine Research Institute

Institut Mondor de Recherche Biomédicale

Hôpital Henri Mondor

Hôpital Albert Chenevier

Roger Le Grand

Function : Correspondent author
PersonId : 834787

Connectez-vous pour contacter l'auteur

Immunologie des maladies virales, auto-immunes, hématologiques et bactériennes

Abstract

Abstract Achieving sufficient worldwide vaccination coverage against SARS-CoV-2 will require additional approaches to currently approved viral vector and mRNA vaccines. Subunit vaccines may have distinct advantages when immunizing vulnerable individuals, children and pregnant women. Here, we present a new generation of subunit vaccines targeting viral antigens to CD40-expressing antigen-presenting cells. We demonstrate that targeting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein to CD40 (αCD40.RBD) induces significant levels of specific T and B cells, with long-term memory phenotypes, in a humanized mouse model. Additionally, we demonstrate that a single dose of the αCD40.RBD vaccine, injected without adjuvant, is sufficient to boost a rapid increase in neutralizing antibodies in convalescent non-human primates (NHPs) exposed six months previously to SARS-CoV-2. Vaccine-elicited antibodies cross-neutralize different SARS-CoV-2 variants, including D614G, B1.1.7 and to a lesser extent B1.351. Such vaccination significantly improves protection against a new high-dose virulent challenge versus that in non-vaccinated convalescent animals.

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Life Sciences [q-bio]

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s41467-021-25382-0.pdf (1.26 Mo)

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https://hal.inria.fr/hal-03481526

Submitted on : Friday, December 17, 2021-11:09:30 AM

Last modification on : Tuesday, January 17, 2023-4:24:03 PM

Long-term archiving on: Friday, March 18, 2022-6:45:40 PM

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hal-03481526 , version 1 (17-12-2021)

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Attribution - CC BY 4.0

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HAL Id : hal-03481526 , version 1
DOI : 10.1038/s41467-021-25382-0
PUBMED : 34471122
PUBMEDCENTRAL : PMC8410935

Cite

Romain Marlin, Veronique Godot, Sylvain Cardinaud, Mathilde Galhaut, Severin Coleon, et al.. Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques. Nature Communications, 2021, 12, pp.5215. ⟨10.1038/s41467-021-25382-0⟩. ⟨hal-03481526⟩

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Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

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