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Adsorption-induced fibronectin aggregation and fibrillogenesis

Hugues Berry 1 Delphine Pellenc 2 Olivier Gallet 2
1 ALCHEMY - Architectures, Languages and Compilers to Harness the End of Moore Years
LRI - Laboratoire de Recherche en Informatique, UP11 - Université Paris-Sud - Paris 11, Inria Saclay - Ile de France, CNRS - Centre National de la Recherche Scientifique : UMR8623
Abstract : Fibronectin (Fn), a high molecular weight glycoprotein, is a central element of extracellular matrix architecture that is involved in several fundamental cell processes. In the context of bone biology, little is known about the influence of the mineral surface on fibronectin supramolecular assembly. We investigate fibronectin morphological properties induced by its adsorption onto a model mineral matrix of hydroxyapatite (HA). Fibronectin adsorption onto HA spontaneously induces its aggregation and fibrillation. In some cases, fibronectin fibrils are even found connected into a dense network that is close to the matrix synthesized by cultured cells. Fibronectin adsorption-induced self-assembly is a time-dependant process that is sensitive to bulk concentration. The N-terminal domain of the protein, known to be implicated in its self-association, does not significantly inhibit the protein self-assembly while increasing ionic strength in the bulk alters both aggregation and fibrillation. The addition of a non-ionic surfactant during adsorption tends to promote aggregation with respect to fibrillation. Ultimately, fibronectin fibrils appear to be partially structured like amyloid fibrils as shown by thioflavine T staining. Taken together, our results suggest that there might be more than one single organization route involved in fibronectin self-assembly onto hydroxyapatite. The underlying mechanisms are discussed with respect to Fn conformation, Fn/surface and Fn/Fn interactions, and a model of fibronectin fibrillogenesis onto hydroxyapatite is proposed.
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https://hal.inria.fr/inria-00001063
Contributor : Hugues Berry <>
Submitted on : Monday, January 30, 2006 - 10:28:38 AM
Last modification on : Wednesday, December 2, 2020 - 3:43:16 AM

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Hugues Berry, Delphine Pellenc, Olivier Gallet. Adsorption-induced fibronectin aggregation and fibrillogenesis. Journal of Colloid and Interface Science, Elsevier, 2006, 298, pp.132-144. ⟨inria-00001063⟩

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