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Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons

Fabrice Guillemot Charles Auffray Marie-Dominique Devignes 1
1 LANGUE ET DIALOGUE - Human-machine dialogue with a significant language component
INRIA Lorraine, LORIA - Laboratoire Lorrain de Recherche en Informatique et ses Applications
Abstract : A limited number of genes, including the human brain-derived neutrotrophic factor (BDNF) gene, have been identified in the human chromosome 11p14 region. Since this area is involved in a genetic disorder (WAGR syndrome) and because of interest in studying the regulation of the human BDNF gene, we have established a detailed transcript map of a 810-kb region clone in a yeast artificial chromosome (YAC), corresponding to a portion of this genomic locus. A set of nested deletion mutants has been generated to map genes at a mean resolution of 75kb. Four genic markers from available mapping databases have been mapped on the YAC.Ten potential novel human exons have been isolated by a 3' terminal exon trapping procedure directly applied to purified YAC DNA. Most of these exons display polyadenylation signals and they all yield positive signals in RT-PCR experiments, confirming their status of transcribed sequences. The BDNF gene is now co-localised with three other genes on a 120 kb DNA fragment.
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Submitted on : Tuesday, September 26, 2006 - 8:39:56 AM
Last modification on : Friday, February 26, 2021 - 3:28:04 PM


  • HAL Id : inria-00098891, version 1



Fabrice Guillemot, Charles Auffray, Marie-Dominique Devignes. Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons. European Journal of Human Genetics, Nature Publishing Group, 1999, 7 (4), pp.487-495. ⟨inria-00098891⟩



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