Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons

Fabrice Guillemot Charles Auffray Marie-Dominique Devignes 1
1 LANGUE ET DIALOGUE - Human-machine dialogue with a significant language component
INRIA Lorraine, LORIA - Laboratoire Lorrain de Recherche en Informatique et ses Applications
Abstract : A limited number of genes, including the human brain-derived neutrotrophic factor (BDNF) gene, have been identified in the human chromosome 11p14 region. Since this area is involved in a genetic disorder (WAGR syndrome) and because of interest in studying the regulation of the human BDNF gene, we have established a detailed transcript map of a 810-kb region clone in a yeast artificial chromosome (YAC), corresponding to a portion of this genomic locus. A set of nested deletion mutants has been generated to map genes at a mean resolution of 75kb. Four genic markers from available mapping databases have been mapped on the YAC.Ten potential novel human exons have been isolated by a 3' terminal exon trapping procedure directly applied to purified YAC DNA. Most of these exons display polyadenylation signals and they all yield positive signals in RT-PCR experiments, confirming their status of transcribed sequences. The BDNF gene is now co-localised with three other genes on a 120 kb DNA fragment.
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Article dans une revue
European Journal of Human Genetics, Nature Publishing Group, 1999, 7 (4), pp.487-495
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Soumis le : mardi 26 septembre 2006 - 08:39:56
Dernière modification le : lundi 25 juin 2018 - 11:06:02

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  • HAL Id : inria-00098891, version 1

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Fabrice Guillemot, Charles Auffray, Marie-Dominique Devignes. Detailed transcript map of a 810-kb region at 11p14 involving identification of 10 novel human 3' exons. European Journal of Human Genetics, Nature Publishing Group, 1999, 7 (4), pp.487-495. 〈inria-00098891〉

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