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Dynamic I-V curves are reliable predictors of naturalistic pyramidal-neuron voltage traces.

Laurent Badel 1 Sandrine Lefort 2 Romain Brette 3 Carl C H Petersen 2 Wulfram Gerstner 1 Magnus J E Richardson 1, 4 
3 ODYSSEE - Computer and biological vision
DI-ENS - Département d'informatique - ENS Paris, CRISAM - Inria Sophia Antipolis - Méditerranée , ENS-PSL - École normale supérieure - Paris, Inria Paris-Rocquencourt, ENPC - École des Ponts ParisTech
Abstract : Neuronal response properties are typically probed by intracellular measurements of current-voltage (I-V) relationships during application of current or voltage steps. Here we demonstrate the measurement of a novel I-V curve measured while the neuron exhibits a fluctuating voltage and emits spikes. This dynamic I-V curve requires only a few tens of seconds of experimental time and so lends itself readily to the rapid classification of cell type, quantification of heterogeneities in cell populations, and generation of reduced analytical models. We apply this technique to layer-5 pyramidal cells and show that their dynamic I-V curve comprises linear and exponential components, providing experimental evidence for a recently proposed theoretical model. The approach also allows us to determine the change of neuronal response properties after a spike, millisecond by millisecond, so that postspike refractoriness of pyramidal cells can be quantified. Observations of I-V curves during and in absence of refractoriness are cast into a model that is used to predict both the subthreshold response and spiking activity of the neuron to novel stimuli. The predictions of the resulting model are in excellent agreement with experimental data and close to the intrinsic neuronal reproducibility to repeated stimuli.
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Submitted on : Thursday, October 8, 2009 - 12:16:29 PM
Last modification on : Thursday, March 17, 2022 - 10:08:29 AM




Laurent Badel, Sandrine Lefort, Romain Brette, Carl C H Petersen, Wulfram Gerstner, et al.. Dynamic I-V curves are reliable predictors of naturalistic pyramidal-neuron voltage traces.. Journal of Neurophysiology, American Physiological Society, 2008, 99 (2), pp.656-66. ⟨10.1152/jn.01107.2007⟩. ⟨inria-00422696⟩



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