Complex wavelet regularization for 3D confocal microscopy deconvolution

Mikael Carlavan 1 Laure Blanc-Féraud 1
1 ARIANA - Inverse problems in earth monitoring
CRISAM - Inria Sophia Antipolis - Méditerranée , SIS - Signal, Images et Systèmes
Abstract : Confocal microscopy is an increasingly popular technique for 3D imaging of biological specimens which gives images with a very good resolution (several tenths of micrometers), even though degraded by both blur and Poisson noise. Deconvolution methods have been proposed to reduce these degradations, some of them being regularized on a Total Variation prior, which gives good results in image restoration but does not allow to retrieve the thin details (including the textures) of the specimens. We first propose here to use instead a wavelet prior based on the Dual-Tree Complex Wavelet transform to retrieve the thin details of the object. As the regularizing prior efficiency also depends on the choice of its regularizing parameter, we secondly propose a method to select the regularizing parameter following a discrepancy principle for Poisson noise. Finally, in order to implement the proposed deconvolution method, we introduce an algorithm based on the Alternating Direction technique which allows to avoid inherent stability problems of the Richardson-Lucy multiplicative algorithm which is widely used in 3D image restoration. We show some results on real and synthetic data, and compare these results to the ones obtained with the Total Variation and the Curvelets priors. We also give preliminary results on a modification of the wavelet transform allowing to deal with the anisotropic sampling of 3D confocal images.
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[Research Report] RR-7366, INRIA. 2010
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Mikael Carlavan, Laure Blanc-Féraud. Complex wavelet regularization for 3D confocal microscopy deconvolution. [Research Report] RR-7366, INRIA. 2010. 〈inria-00509447v2〉

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