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, Ajouter 500 µl de CIA (96 % Chloroforme 4 % Isoamyle Alcool) ? Mélanger manuellement pendant 2 minutes ? Centrifuger à 4°C et à 15 000 g pendant 10 minutes ? Récupérer la phase aqueuse surnageante

, Précipitation ? Ajouter 250 µl d'isopropanol froid et 1 µg de RNA carrier ? Laisser une nuit au congélateur à, p.20

, Récupération du culot ? Centrifuger à 4°C et à 15 000 g pendant 10 minutes ? Jeter le surnageant ? Ajouter 500 µl d'éthanol 95 % froid ? Centrifuger à 4°C et à 15 000 g pendant 10 minutes ? Jeter le surnageant ? Laisser sécher les tubes sous la hotte ? Re

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, Bouvier (1925) reported six specimens belonging to C. nilotica brachydactyla De Man, 1908 (A. Marche coll.) with a short rostrum armed to the tip on both margins. Specimens are deposited in MNHN (MNHN-IU-2015-1824), View publication stats View publication stats From the Mariana Islands (with no island specified), 2001.

. Keith, Amplification products were generated by an initial denaturation step of 4 min at 94°C followed by 35 cycles of denaturation at 94°C for 30s, annealing at 52°C for 40 s, extension at 72°C for 60 s and a final extension step at 72°C for 7 min. For old collection specimens (Syntypes of C. mertoni), a CTAB protocol was used to extract DNA from pleopods. A shorter fragment of the 16S rRNA (332 bp) was amplified using two newly designed primers: 16S-Car-81F (AGGTAGCATAATAAATAGTC) and 16S-Car-413R (CTGTTATCCCTAAAGTAAC). DNA amplification was performed in 25µl PCR reactions, containing 2.5 mM MgCl2, 0.26 mM of each nucleotide, 0.3 µM of each primer, 1 ng of BSA and 1.5 units of QBIOTAQ polymerase (MPBiomedicals), C. longirostris became C. brachydactyla and C. nilotica became C. mertoni Roux, 1911 in the Pacific. Recently one of us (DCR in March 2015) collected various Caridina from Guam, with some specimens bearing a long rostrum and attributed to C. brachydactyla, whereas others, with a short rostrum, were identified as C. mertoni following the previous identifications made by Leberer & Cai, 2003. Bright (1979) reported C. brachydactyla from Palau among seven Atyid species, depositing them at the USNM (USNM 172589 and USNM 172590), 2003.

(. D. Guam, A. Christopher-rogers, and T. Farahi, Jones coll.) 30/01-03/02/2011 13°28.168'N 144°42.762'E Assan 11-16 national d'Histoire naturelle, Paris: MNHN; Naturhistorisches Museum Basel, Basel: NMB; National Museum of Natural History

R. Van-natuurlijke-historie, Naturalis Biodiversity Center

, ) by tracing vectorial paths on stacks of high-resolution photographs using Adobe Illustrator (CS6) and a WACOM MPTZ-1230 graphic tablet. PCA for some museum specimens. Some museum specimens, for which DNA could not be retrieved for a genetic analysis, but still having all their pereiopods, were measured and included in a PCA with our recent sequenced specimens and old specimens that yielded DNA. We followed the method proposed by Konan et al (2010) using Statistica v.10 software (Statsoft). The same 19 ratios and counts used in previous work (de Mazancourt et al, 2017) were used for the analysis, ZRC Abbreviations for morphological analyses. The following abbreviations are used in the present text: cl, carapace length (mm): measured from the post-orbital margin to the posterior margin of the carapace. P1: first pereiopod. P2: second pereiopod. P3: third pereiopod. P5: fifth pereiopod. Pl1: first pleopod, 2004.

. Indonesia, Aru Island, Syntypes C. mertoni. NMB 693a one male cl 2.7 mm; NMB 693b one male 3.1 mm

, Lectotype C. brachydactyla ZMA 977 one ovigerous female cl 4.8 mm, Sulawesi, Paralectotypes ZMA 2552 two ovigerous female cl, NMB 693c one non-ovigerous female

, Maximum likelihood phylogenetic tree (16S mtDNA) of the specimens. CAXXXX numbers are lab IDs of the specimens. Results Phylogenetic analyses. A total of 31 recent specimens were sequenced, including 23 of the new species (10 from Guam, 13 from Palau), 5 recent specimens of C

C. Not and . Brachydactyla, 3 mm (MNHN-IU-2017-2105 DNA: CA1544) and 1? ovig cl 4.5 mm (MNHN-IU-2017-2106 DNA: CA1538). BPBM 4227 1? ovig cl 4.4 mm, USNM 172589: 1? cl 2.5 mm and 1? cl 3.0 mm; USNM 172590: 1? cl 5.5 mm. GUAM. Assan River: DCR (D. Christopher Rogers collections) 9? cl 2.7 mm (MNHN-IU-2017-2098 DNA: CA1535), 3.5 mm (MNHN-IU-2017-2099 DNA: CA1536), 2.3 mm (MNHN-IU-2017-2100 DNA: CA1537), 2.7 mm (MNHN-IU-2017-2101 DNA: CA1539), 2.8 mm (MNHN-IU-2017-2102 DNA: CA1540), 2.4 mm (MNHN-IU-2017-2103 DNA: CA1541), 2.2 mm (MNHN-IU-2017-2104 DNA: CA1542), 2.7 mm (MNHN-IU-2017-2102 DNA: CA1543), vol.2, 2011.

, Second pereiopod 2; c. Third pereiopod; d. Fifth pereiopod; e. Dactylus of third pereiopod; f. Dactylus of fifth pereiopod; g. Male first pleopod; h. Male second pleopod; i. Eggs; j. Uropodial diaeresis; k. Pre-anal carina; l. Telson; m., n. Cephalothorax; o. General appearance; p. Right mandible; q. Left mandible; r. First maxilla; s. Second maxilla; t. Third maxilliped; u. Second maxilliped; v. First maxilliped, Zootaxa, vol.4377, issue.1, p.45, 2018.

, A NEW SPECIES OF CARIDINA FROM MICRONESIA Comparative material. -Caridina brachydactyla De Man, 1908.

, Other material: INDONESIA: C. brachydactyla: NMB 1054a, Bali, 1? cl 5.8 mm. Sulawesi, Palopo, Macaui, 63.10, 2? cl 2.7-3.7 mm; 1? ovig cl 4.3 mm and 1?, cl 4.8 mm; Palopo, Tojo, 64.10.3 1? ovig cl 4.0 mm. -Caridina mertoni Roux, 1911 Type material: INDONESIA; Cotypes: Grand Kai, RMNH 2552, 2? ovig (cl 5.3-5.4 mm)

. Nmbc, MNHN-IU-2015-1819 1? cl 3.8 mm

, 2108 DNA: CA1507), 3.7 mm (MNHN-IU-2017-2109 DNA: CA1505), 1? ovig cl 3.9 mm, 1977.

. Hong-kong, 3? cl 2.8-3.5 mm and 1? cl 4.2 mm

-. Kemp, 1? cl 3.4 mm; . Other material: SINGAPORE; Tanglin (incorrectly spelt Tangtum in NHM register and on label, Type material: Caridina brachydactyla subsp. peninsularis Kemp, p.1, 1916.

, 7-1.5 of cl, reaching to scaphocerite apex, armed dorsally with 16-24 teeth, distal unarmed portion 0.1-0.7 times that of armed portion, with one or two subapical teeth, 1-4 teeth on carapace posterior to orbital margin, ventral margin with 4-15 teeth. Number of dorsal teeth before the most proximal ventral tooth 10-17, Description. Cephalothorax. Rostrum (Fig. 4m): very variable in length, 0

, Antennular spine acute, placed slightly below orbital angle. Pterygostomian margin rectangular. Eyes well developed, anterior end reaching to 0.7 length of antennular peduncle basal antennomere

, Upper lacinia elongate, with medial margin bearing a number of distinct teeth, palp setose. Maxilla (Fig. 4s) upper and middle endite with marginal and submarginal plumose setae. Lower endite with simple setae; palp narrow, shorter than upper endite cleft with a few setae. Scaphognathite fringed with long setae, tapering posteriorly with some long, curved setae at posterior end. First maxilliped (Fig. 4v) endopodite ultimate segment medial margin with long denticulate setae, lower portion with transverse rows of simple setae. Palp elongate, setose. Exopod flagellum long and narrow distally with marginal plumose setae. Caridean lobe large, with marginal setae, Antennular peduncle (Fig. 4m, 4n, cephalothorax view, and 4o, general view) 0of antennular peduncle basal antennomere. Scaphocerite extending slightly beyond antennular peduncleapex, length about 2.8 times width

C. Kemp, This new species displays a variable rostrum length among specimens. When the rostrum is short the general appearance is like C. mertoni, whereas when the rostrum is long the general appearance is of C. brachydactyla. In C. variabilis n. sp. the antennal spine is placed below the orbital angle, the P5 dactylus is biunguiculate with 13-30 setae vs. 32-42 in C. brachydactyla and there is a very large propodus seta, whereas in C. mertoni, the antennal spine is somewhat fused with the orbital angle, possesses an unguiculate dactylus with 30-40 setae and has no prominent propodus seta. Caridina variabilis n. sp. differs from C. brachydactyla by the absence of spine on the preanal carina, a shorter distal unarmed portion of the rostrum 0.1-0.7 (vs 0.6-1.6) times that of armed portion, mm. Type Locality. Palau, Babeladob Island, Tabecheding River, 07°27.169N, 134°31.748E. Habitat. This species is found in rivers from lower to higher elevations in flowing water between riffles, 1918.

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, Avec plus de 300 espèces décrites, il s'agit du genre le plus diversifié de l'infra--ordre des Caridea, avec une systématique extrêmement confuse et compliquée. Au sein de ce genre, deux complexes d'espèces sont particulièrement bien représentés dans les systèmes insulaires de l'Indo--Pacifique, le complexe Caridina nilotica et , permettant d'obtenir 1682 séquences auxquelles s'ajoutent 32 génomes mitochondriaux complets et 97 partiels, mettant en évidence 43 espèces nouvelles, certaines décrites au cours de la thèse. Les relations phylogénétiques entre les espèces des deux complexes ont été reconstruites à partir d'un grand jeu de données moléculaires, permettant de montrer que les complexes sont des groupes monophylétiques avec des différences en terme d'habitats occupés. Enfin, la faisabilité de l'étude sclérochronologique de l'amphidromie chez une espèce du complexe C. weberi (C. multidentata) a été testée sur la cuticule du pédoncule oculaire, Résumé Les cours d'eau des îles tropicales abritent des organismes qui ont développé un cycle de vie diadrome, partagé entre une phase adulte en eau douce et une phase larvaire marine : l'amphidromie. Parmi ces organismes, dans la zone Indo--Pacifique, on trouve les crevettes du genre Caridina H. Milne Edwards, 1837