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Synthèse de nanoparticules d‘oxydes métalliques et leur activité antibactérienne

Abstract : This thesis aims are the synthesis of nanoparticles of metal oxides (CuO, ZnO and TiO₂) by soft chemistry "sol-gel" for applications in the biomedical field. Nanoparticles of ZnO and CuO are prepared from acetate precursors, and TiO₂ is produced in a fast micromixer reactor allowing size control of nanoparticles. Thin layers have been developed by dip-coating process.Metal oxides antibacterial activity has been studied on strains from European Pharmacopoeia; Gram-positive bacterium: Staphylococcus aureus, two Gram-negative bacteria: Escherichia coli and Pseudomonas aeruginosa and a fungus : Candida albicans. Different methods have been adopted for antibacterial activity evaluation in vitro: disk diffusion and well diffusion,bioluminescence (ATP measurements) and stall plate technique. Tests carried out showed an antibacterial activity of nanoparticles of ZnO and CuO in amorphous and crystalline form, and only in crystalline form for TiO₂. A mathematical model has been developed based on the results of the antibacterial tests and kinetic evolution of the bacterial population. It concluded that bacterial suppression is more efficient during the exponential phase, while its efficiency is lower during the stationary phase and latency. Cytotoxicity tests of NPs in suspension on gingival fibroblasts showed a decrease in cell viability, in part due to the presence of the solvent.
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Submitted on : Saturday, January 23, 2021 - 5:52:08 PM
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Rania Dadi. Synthèse de nanoparticules d‘oxydes métalliques et leur activité antibactérienne. Matériaux. Université Paris-Nord - Paris XIII, 2019. Français. ⟨NNT : 2019PA131039⟩. ⟨tel-03119316⟩



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