We consider the separation of sources when only one movable sensor is available to record a set of mixtures at distinct locations. A single mixture signal is acquired, which is firstly segmented. Then, based on the assumption that the underlying sources are temporally periodic, we align the resulting signals and form a measurement vector on which source separation can be performed. We demonstrate that this approach can successfully recover the original sources both when working with simulated data, and for a real problem of heart sound separation.