Genomic approach to study floral development genes in Rosa sp.

Annick Dubois 1 Arnaud Remay 2 Olivier Raymond 1 Sandrine Balzergue 3 Aurélie Chauvet 1 Marion Maene 1 Yann Pécrix 4 Shu-Hua Yang 1 Julien Jeauffre 5 Tatiana Thouroude 2 Véronique Boltz 1 Marie-Laure Martin-Magniette 3 Stéphane Janczarski 1 Fabrice Legeai 6, 7, 8 Jean-Pierre Renou 3 Philippe Vergne 9 Manuel Le Bris 4 Fabrice Foucher 10 Mohammed Bendahmane 1
1 RDP - Reproduction et développement des plantes
2 Unité mixte de recherche génétique et horticulture Genhort
3 URGV - Unité de recherche en génomique végétale
4 IMEP - Institut Méditerranéen d'Ecologie et de Paléoécologie
5 GENHORT - Génétique et Horticulture
6 BIO3P - Biologie des organismes et des populations appliquées à la protection des plantes
7 SYMBIOSE - Biological systems and models, bioinformatics and sequences
IRISA - Institut de Recherche en Informatique et Systèmes Aléatoires, Inria Rennes – Bretagne Atlantique
8 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
9 LaMCoS - Laboratoire de Mécanique des Contacts et des Structures [Villeurbanne]
10 Service de Gynécologie et Obstétrique [Rennes]
Abstract : Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower.
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Article dans une revue
PLoS ONE, Public Library of Science, 2011, 6 (12), pp.e28455. 〈10.1371/journal.pone.0028455〉
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https://hal.inria.fr/hal-00753396
Contributeur : Fabrice Legeai <>
Soumis le : lundi 19 novembre 2012 - 11:09:23
Dernière modification le : mercredi 16 mai 2018 - 11:24:04

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INRIA | IRISA | RDP | ENS-LYON | UNAM | IMEP | INRA | IRISA-D7 | INSA-LYON | LAMCOS | UNIV-AMU | UR1-UFR-ISTIC | UNIV-LYON1 | UNIV-RENNES

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Annick Dubois, Arnaud Remay, Olivier Raymond, Sandrine Balzergue, Aurélie Chauvet, et al.. Genomic approach to study floral development genes in Rosa sp.. PLoS ONE, Public Library of Science, 2011, 6 (12), pp.e28455. 〈10.1371/journal.pone.0028455〉. 〈hal-00753396〉

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