Evidence for the Existence of Elaborate Enzyme Complexes in the Paleoarchean Era

Abstract : : Due to the lack of macromolecular fossils, the enzymatic repertoire of extinct species has remained largely unknown to date. In an attempt to solve this problem, we have characterized a cyclase subunit (HisF) of the imidazole glycerol phosphate synthase (ImGP-S), which was reconstructed from the era of the last universal common ancestor of cellular organisms (LUCA). As observed for contemporary HisF proteins, the crystal structure of LUCA-HisF adopts the (βα)8-barrel architecture, one of the most ancient folds. Moreover, LUCA-HisF (i) resembles extant HisF proteins with regard to internal 2-fold symmetry, active site residues, and a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to that of contemporary (βα)8-barrel enzymes, (iii) displays high catalytic activity, and (iv) forms a stable and functional complex with the glutaminase subunit (HisH) of an extant ImGP-S. Furthermore, we show that LUCA-HisF binds to a reconstructed LUCA-HisH protein with high affinity. Our findings suggest that the evolution of highly efficient enzymes and enzyme complexes has already been completed in the LUCA era, which means that sophisticated catalytic concepts such as substrate channeling and allosteric communication existed already 3.5 billion years ago.
Type de document :
Article dans une revue
Journal of the American Chemical Society, American Chemical Society, 2014, pp.122-9. 〈10.1021/ja4115677〉
Liste complète des métadonnées

Littérature citée [2 références]  Voir  Masquer  Télécharger

https://hal.inria.fr/hal-00924047
Contributeur : Samuel Blanquart <>
Soumis le : lundi 1 décembre 2014 - 13:40:21
Dernière modification le : vendredi 8 janvier 2016 - 01:07:40
Document(s) archivé(s) le : lundi 2 mars 2015 - 13:31:23

Fichier

LUCAHisF_Manuscript_JACS_revis...
Fichiers produits par l'(les) auteur(s)

Identifiants

Citation

Bernd Reisinger, Josef Sperl, Alexandra Holinski, Veronika Schmid, Chitra Rajendran, et al.. Evidence for the Existence of Elaborate Enzyme Complexes in the Paleoarchean Era. Journal of the American Chemical Society, American Chemical Society, 2014, pp.122-9. 〈10.1021/ja4115677〉. 〈hal-00924047〉

Partager

Métriques

Consultations de
la notice

168

Téléchargements du document

232