Dendritic cell-based therapeutic vaccine elicits polyfunctional HIV-specific T-cell immunity associated with control of viral load.

Abstract : Efforts aimed at restoring robust immune responses limiting human immunodeficiency virus (HIV)-1 replication therapeutically are warranted. We report that vaccination with dendritic cells generated ex vivo and loaded with HIV lipopeptides in patients (n = 19) on antiretroviral therapy was well tolerated and immunogenic. Vaccination increased: (i) the breadth of the immune response from 1 (1-3) to 4 (2-5) peptide-pool responses/patient (p = 0.009); (ii) the frequency of functional T cells (producing at least two cytokines among IFN-γ, TNF-α, and IL-2) from 0.026 to 0.32% (p = 0.002) and from 0.26 to 0.35% (p = 0.005) for CD4(+) and CD8(+) T cells, respectively; and (iii) the breadth of cytokines secreted by PBMCs upon antigen exposure, including IL-2, IFN-γ, IL-21, IL-17, and IL-13. Fifty percent of patients experienced a maximum of viral load (VL) 1 log10 lower than the other half following antiretroviral treatment interruption. An inverse correlation was found between the maximum of VL and the frequency of polyfunctional CD4(+) T cells (p = 0.007), production of IL-2 (p = 0.006), IFN-γ (p = 0.01), IL-21 (p = 0.006), and IL-13 (p = 0.001). These results suggest an association between vaccine responses and a better control of viral replication. These findings will help in the development of strategies for a functional cure for HIV infection.
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European Journal of Immunology, Wiley-VCH Verlag, 2014, 44 (9), pp.2802-10
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https://hal.inria.fr/hal-01108024
Contributeur : Sandrine Darmigny <>
Soumis le : jeudi 22 janvier 2015 - 09:40:55
Dernière modification le : jeudi 15 mars 2018 - 10:12:30

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  • HAL Id : hal-01108024, version 1
  • PUBMED : 25042008

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Yves Lévy, Rodolphe Thiébaut, Monica Montes, Christine Lacabaratz, Louis Sloan, et al.. Dendritic cell-based therapeutic vaccine elicits polyfunctional HIV-specific T-cell immunity associated with control of viral load.. European Journal of Immunology, Wiley-VCH Verlag, 2014, 44 (9), pp.2802-10. 〈hal-01108024〉

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