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Detection and Tracking of Golgi Outposts in Microscopy Data

Huei-Fang Yang 1 Chu-Song Chen 1, 2 Xavier Descombes 3 
3 MORPHEME - Morphologie et Images
CRISAM - Inria Sophia Antipolis - Méditerranée , IBV - Institut de Biologie Valrose : U1091, Laboratoire I3S - SIS - Signal, Images et Systèmes
Abstract : Golgi outposts (GOPs) that transport proteins in both the anterograde and retrograde directions play an important role in determining the dendritic morphology in developing neurons. To obtain their heterogeneous motion patterns, we present a data association based framework that first detects the GOPs and then links the detection responses. In the GOP detection stage, we introduce a multi-scale Markov Point Process (MPP) based particle detector that uses multi-scale blobness images obtained by Laplace of Gaussian (LoG) for GOP appearances. This reduces the number of missed detections compared to the use of image intensity for GOP appearances. In the linking stage, we associate detection responses to form reliable tracklets and link the tracklets to form long, complete tracks. As such, high-level information (e.g., motion) is encoded in building the affinity model. We evaluate our approach on the microscopy data sets of dendritic arborization (da) sensory neurons in Drosophila larvae, and the results demonstrate the effectiveness of our method.
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Submitted on : Friday, February 6, 2015 - 4:17:38 PM
Last modification on : Thursday, August 4, 2022 - 4:55:03 PM
Long-term archiving on: : Thursday, May 7, 2015 - 10:16:04 AM


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  • HAL Id : hal-01113626, version 1



Huei-Fang Yang, Chu-Song Chen, Xavier Descombes. Detection and Tracking of Golgi Outposts in Microscopy Data. ISBI - International Symposium on Biomedical Imaging, Apr 2015, Brooklyn, New York, United States. ⟨hal-01113626⟩



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