This paper presents image-based quantitative evaluation of subtle variations in facial wrinkles for the same subject in response to a dermatological treatment. This is a novel application because the time series images of the same subject over a shorter time period of weeks are analyzed as compared to more prevalent inter-person analysis of facial skin/marks. We propose image features based on Gabor filter bank for an accurate quantitative evaluation of variations in facial wrinkles. Since variations in Gabor features are very small on a time period of weeks, we propose a framework to compare image features in key wrinkle sites only while excluding the noise introduced by non-wrinkle sites. The framework consists of finer registration of images using Large Deformation Diffeo-morphic Metric Mapping (LDDMM) and detection of wrinkle sites using Gabor filter bank and morphological image processing. Preliminary experiments show that the framework is useful in calculating variations in Gabor features at detected sites and indicating trends in the response of facial wrinkles to the dermatological treatment.