Lifetime estimation on moving sub-cellular objects in frequency domain FLIM imaging

Abstract : Fluorescence lifetime is usually defined as the average nanosecond-scale delay between excitation and emission of fluorescence. It has been established that lifetime measurement yields numerous indications on cellular processes such as inter-protein and intra-protein mechanisms through fluorescent tagging and Förster resonance energy transfer (FRET). In this area, frequency domain fluorescence lifetime imaging microscopy (FD FLIM) is particularly well appropriate to probe a sample non-invasively and quantify these interactions in living cells. The aim is then to measure fluorescence lifetime in the sample at each location in space from fluorescence variations observed in a temporal sequence of images obtained by phase modulation of the detection signal. This leads to a sensitivity of lifetime determination to other sources of fluorescence variations such as intracellular motion. In this paper, we propose a robust statistical method for lifetime estimation on both background and small moving structures with a focus on intracellular vesicle trafficking.
Complete list of metadatas

Cited literature [32 references]  Display  Hide  Download

https://hal.inria.fr/hal-01246392
Contributor : Charles Kervrann <>
Submitted on : Friday, December 18, 2015 - 4:31:27 PM
Last modification on : Tuesday, May 28, 2019 - 1:41:55 AM
Long-term archiving on : Saturday, April 29, 2017 - 9:52:22 PM

File

FDFLIMMOTION-2015.pdf
Files produced by the author(s)

Identifiers

Citation

Philippe Roudot, Charles Kervrann, Cedric Blouin, Francois Waharte. Lifetime estimation on moving sub-cellular objects in frequency domain FLIM imaging. Journal of the Optical Society of America. A Optics, Image Science, and Vision, Optical Society of America, 2015, 32 (10), pp.15. ⟨10.1364/JOSAA.32.001821⟩. ⟨hal-01246392⟩

Share

Metrics

Record views

529

Files downloads

170