Lifetime estimation on moving sub-cellular objects in frequency domain FLIM imaging

Abstract : Fluorescence lifetime is usually defined as the average nanosecond-scale delay between excitation and emission of fluorescence. It has been established that lifetime measurement yields numerous indications on cellular processes such as inter-protein and intra-protein mechanisms through fluorescent tagging and Förster resonance energy transfer (FRET). In this area, frequency domain fluorescence lifetime imaging microscopy (FD FLIM) is particularly well appropriate to probe a sample non-invasively and quantify these interactions in living cells. The aim is then to measure fluorescence lifetime in the sample at each location in space from fluorescence variations observed in a temporal sequence of images obtained by phase modulation of the detection signal. This leads to a sensitivity of lifetime determination to other sources of fluorescence variations such as intracellular motion. In this paper, we propose a robust statistical method for lifetime estimation on both background and small moving structures with a focus on intracellular vesicle trafficking.
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Soumis le : vendredi 18 décembre 2015 - 16:31:27
Dernière modification le : mercredi 21 mars 2018 - 18:57:05
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Philippe Roudot, Charles Kervrann, Cedric Blouin, Francois Waharte. Lifetime estimation on moving sub-cellular objects in frequency domain FLIM imaging. Journal of the Optical Society of America A, Optical Society of America, 2015, 32 (10), pp.15. 〈〉. 〈10.1364/JOSAA.32.001821〉. 〈hal-01246392〉



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