Boron nitride (BN) is known for high hardness, thermal stability, thermal conductivity, and catalytic action. The aim of this study was to investigate the effect of biofield treatment on physical, structural and spectral properties of BN powder. The control and treated sample of BN powder were characterized by X-ray diffraction (XRD), surface area analysis and Fourier transform infrared spectroscopy (FT-IR). XRD results indicated that biofield treatment had substantially changed the crystallinity of BN powder as compared to control. Apart from the crystallinity, significant changes were also observed in lattice parameter, density and molecular weight of the treated BN powder as compared to control sample. The XRD data confirmed 33.30% increase crystallite size in treated BN powder as compared to control. The surface area data showed 10.33% increment in surface area of treated BN as compared to control. Furthermore, FT-IR spectra revealed that some part of BN may be transformed from hexagonal BN (h-BN) to rhombohedral boron nitride (r-BN), which was corroborated by emergence of new prominent peaks at 1388 cm-1 in treated BN as compared to control sample. These findings suggest that biofield treatment has substantially altered the structural properties and surface area of treated BN powder.